Balls , — Temperature and Growth . 
577 
light on our hypothesis. The first of these is the effect of prolonged 
exposure to ‘ sub- maximal ’ temperatures. 
The effect of such exposure on the morphology of the fungus, and 
on the time required for the completion of staling, has been described 
previously. We now proceed to note the effect on the stopping-point. 
A difficulty in the experimental method might be noted here in 
order to excuse the inaccurate temperatures employed; owing to the 
erratic gas pressure in the laboratory mains, and the absence of a pressure- 
SOX 
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12-10 
Curve 3. Inoculated tube stored at 34~35§°C. for twenty-four hours. Curve represents the 
fastest hypha obtainable. 
regulator, it is not possible to keep the Hearson incubator at a perfectly 
constant temperature. A nominal temperature of 33 0 C. is actually one of 
32-34° C. 
The curve plotted from a culture which has been stored at 33 0 C. 
before being transferred to the damp chamber for study is similar to that 
obtained in the standard cultures, except that the rate of growth is slower, 
and that the stopping-point is lowered . 1 On account of the irregularity 
1 Curve 3, 
