582 Balls.— Temperature and Growth. 
In this connexion it may be pointed out that whereas fresh media either 
give the same stopping-point in duplex curves (Curve 2), or else a depression 
due to staling, the artificially staled cultures commonly show an increase in 
the stopping-point when duplicated ; in one case it rose from 31-0° C. to 
33*5° C. and in another from 29*5° C. to 37*0° C. This would seem to 
indicate that in the thin film of solution in the damp chamber the body 
1 x* is even more readily decomposed than in the large tubes. Further, 
it is possible that some conditions which protect it from decomposition 
exist in the cells of the organism. 
The series of results given below is typical of the data obtained, and 
demonstrates all the important points. 
Medium from a large flask culture, almost stale ; transferred to a series 
of tubes, and heated at ioo° C. in the steam sterilizer for thirty minutes. 
This solution was then treated in various ways. 
(a) Inoculated and tested — growth almost negligible, too slight to 
enable any determination of the stopping-point to be made. In the tube it 
grew about five millimetres in ten days. 
(b) Boiled for four hours under a reflux condenser, and for another hour 
in the sterilizer; growth of fungus free, with a stopping-point at 32-5 °C. 
Curve 4. 1 
(c) Mixed with own bulk of water, i.e. 50 per cent, solution. 
Tested directly . 
. . 2 9 -5°C. 
Boiled in sterilizer for half an hour 
. . 35 -o°C. 
Boiled for another half hour . 
. . 38-0° C. 
Diluted with water to 25 per cent. 
Tested directly . 
. . 3 i.o°C. 
Boiled for half an hour . 
• • 33'5°C. 
Boiled for two hours . 
■ 37-4° C. 
1 Page 580. 
