DR. A. GAMGrEE ON THE ACTION OE NITRITES ON BLOOD. 
605 
in gudgeons fixed in the sides of the tin box E F, G H. The box ABCD is divided by 
a central partition, K T, into two equal compartments. Into the bottom of the box are 
fitted, one in each side, the valves v, v', which are so constructed that when either half 
of the box is dependent, the valve of that side opens. The apparatus is brought beneath 
a stream of water so that the water can enter one half of the box a little to the side of 
the central partition; when a certain amount of water has accumulated, that side 
of the box more than balances the other, and swings over with a powerful jerk ; this 
movement, however, brings the opposite side of the box under the tap, and this 
commences to fill. In the meantime the valve in the side first filled is opened and the 
water is escaping. Thus the box swings alternately from side to side. Supports are 
attached to either end of the box for supporting the absorption-tubes containing the 
blood and gas. If the apparatus be well constructed, the two tubes are placed in exactly 
the same circumstances, each receiving the most thorough shake several times in a minute. 
(«) 
25 cub. centims. of defibrinated and well-arterial- 
ized blood of the Sheep were diluted with 10 cub. 
centims. of distilled water. 25 cub. centims. of this 
mixture of blood and water was placed in a tube of 
the capacity of 55 cub. centims., which was imme- 
diately corked. 
The tube was tied to one of the supports of the 
shaking machine at 4.30 p.m. of one day, and the 
process of agitation continued until 1.15 on the fol- 
lowing day. During this time the tubes received 
1944 shakes. 
After the air had been in contact (for 22 hours 
and 45 minutes) with the blood, it was decanted by 
means of Professor Miller’s gas-pipette and analyzed. 
Yol. of gas taken . . . 154 vols. 
After absorption of C0 2 . 152 „ 
After addition of H . . 246-8 „ 
After explosion . . . 155-5 ,, 
Composition of gas in 100 parts. 
C0 2 . . . . 1-29 
O . . . . 19-76 
IT .... . 78-95 
(&) 
The same quantity of blood as was used in (a) was 
mixed with 10 cub. centims. of distilled water con- 
taining 0-1 grm. of KN0 2 in solution. 25 cub. cen- 
tims. of this mixture of blood and solution of nitrite 
of potassium was placed in a tube of the capacity of 
55 cub. centims., which was immediately corked. 
The tube 6 was treated in all respects like the 
tube a. 
After the air had been in contact with the blood for 
22 hours and 45 minutes, it was decanted by means 
of Professor Miller’s gas-pipette and analyzed. 
Yol. of gas taken . . . 159 vols. 
After absorption of C0 2 . 159 „ 
After addition of H . . 245-3 „ 
After explosion . . . 145-2 ,, 
Composition of gas in 100 parts. 
C0 2 . . . . o-oo A 
O . . . . 20-99 
N .... 79-01 
100-00 
100-00 
