608 
DE. A. GAMGEE ON THE ACTION OE NITEITES ON BLOOD. 
Obs. II. 25 cub. centims. of well-arterialized Sheep’s blood 
centims, of distilled water containing 0"03 grm. of KN0 2 in 
mixed were allowed to remain exposed to the air ; then after 
20 cub. centims. of the mixture were brought in contact with a 
were treated with 3 cub. 
solution, and after being 
an interval of two hours 
measured volume of car- 
The carbonic oxide gas, which was prepared by the action of sulphuric acid upon 
formiate of magnesium, was found, on analysis, to be perfectly 
The blood was allowed to remain in contact with the gas for 
measured, and analyzed. 
Carbonic oxide taken measured .... 
After contact with blood for 21 hours . 
After absorption with potash 
After absorption with pyrogallic acid 
pure. 
21 hours, then decanted. 
315- 9 vols. 
316- 2 „ 
314-8 „ 
314-8 „ 
Composition in 100 parts. 
Carbonic acid 0‘44 
Carbonic oxide and nitrogen . . . 99*56 
100-00 
Carbonic oxide taken =315-9 vols. = 35T9 cub. centims. at 0° C. and 0 m- 76 
Carbonic acid exhaled= 1*4 vol. = 0-156 „ ,, „ 
In the above experiment a mixture of blood and nitrite containing 17*8 cub. centims. 
of blood furnished on agitation with 35T9 cub. centims. of CO, no trace of oxygen gas! 
Obs. III. 50 cub. centims. of defibrinated and well-arterialized blood of the Sheep 
were treated with a few drops of an alcoholic solution of nitrite of amyl. In a few 
minutes the chocolate coloration was very marked, and the spectrum had the character 
of that of blood treated with nitrites. 
Carbonic oxide was prepared by the action of sulphuric acid on formiate of magnesium. 
The gas was analyzed and found to be perfectly pure. 20 cub. centims. of the blood 
acted upon by nitrite of amyl were brought in contact with a measured volume of it, 
and the gas and blood having been mixed by agitation, were allowed to remain in contact 
for twenty hours. 
Volume of blood taken .... 
Carbonic oxide measured .... 
After contact with blood and nitrite 
After absorption of carbonic acid . 
After absorption of oxygen . . . 
20 cub. centims. 
387-9 vols. 
.398-4 „ 
391-1 „ 
390-57 „ 
Composition of gas in 100 parts. 
Oxygen 0*38 
Carbonic acid 1-58 
Carbonic oxide and nitrogen . . 98-04 
100-00 
