yS West. — On Stigeosporium Marattiacearum and 
Both hand- and microtomed sections were cut ; the former were found 
useful for demonstrating the grosser features, whilst the latter were necessary 
for determining the finer details. 
A considerable number of stains and reagents were used, as it was found 
necessary to employ a variety of staining methods in order to successfully 
differentiate the younger parts (especially haustoria) of the fungal mycelium 
from the tissues of the host-plant. 
Fairly good results were obtained by the use of Durand’s 1 haemato- 
xylin-eosin combination for differential staining, but the cotton blue + lactic 
acid method recommended by Jones, Giddings, and Lutman (27, p. 30 , 
foot-note) did not give satisfactory results. 
The following stains were also employed either singly or in combina- 
tion : Kleinenberg’s haematoxylin, safranin, azo-blue, picro-nigrosin, methy- 
lene blue, and Congo red. 
For determining the chemical nature of the cell-walls, &c., various 
reagents, including chlor-zinc-iodide, H 2 S0 4 , iodine, phloroglucin + HC1, 
osmic acid, alkannin, Sudan III, Congo red, and ruthenium red were found 
useful. 
The sections were mounted in Canada balsam or in glycerine jelly. 
A. On the Endophyte of the Roots of Angiopteris evecta, 
Hoffm., Archangiopteris Henryi, Chr. et Gies., Kaulfussia 
AESCULIFOLIA, BL., AND MARATTIA COOPERI, MRE. 
The roots of the first order of all genera and species of Marattiaceae 
are relatively robust and frequently branch in a monopodial manner at some 
distance from the caudex. The roots of the second and higher orders are 
usually long and fibrous, and are provided with multicellular root-hairs 
(r.h., Text-fig. 9 ; PI. Ill, Fig. 3 ), which are rather abundant on the fine 
threadlike terminal rootlets. 
In all the above-named genera the roots are characterized by the com- 
plete absence of a cortical zone of stereome (cf. Danaea ), although isolated 
‘ stone-cells ’ are not infrequently met with near the periphery of the larger 
roots. The ground-tissue of the cortex consists of typical large paren- 
chymatous cells, many of which are packed with starch. 
The endophyte, a brief description of which the present writer (44) has 
already published, is of general occurrence in the primary and earlier 
adventitious roots, but its presence appears to be less regular in the later 
roots of each genus. 
It seldom occurs in the aerial portion of the main roots, and is invariably 
absent from the terminal (i. e. meristematic) region of all roots. 
1 Durand, E. J. : The Differential Staining of Intercellular Mycelium. Phytopathology, i, 1911, 
p. 129. 
