Allen . — The Spermatogenesis of Poly trichum juniper mum. 283 
is represented, if at all, only by a faint tinge, although it was plainly visible 
before fixation. Doubtless the substances surrounding and separating the 
antherozoids were largely dissolved in the liquids through which the 
preparation was passed. The antherozoid shown in Fig. 58 is beginning to 
free itself, probably having pushed its anterior end beyond the boundary of 
its enclosing vesicle ; and that in Fig. 59 is quite free. The difficulty that 
the antherozoids of Polytrichum apparently find in freeing themselves from 
the enclosing vesicles has been noted by other observers (most recently by 
Walker, 1913), in contrast with the readiness with which the antherozoids of 
other Mosses are set at liberty. It may well be that the conditions in this 
respect are different in nature from those which obtain in a drop of water 
under observation in the laboratory, 
Each antherozoid in motion shows under a comparatively low power of 
the microscope a refractive spot ; with the oil-immersion objective this spot 
is seen to be a hollow sphere containing many granules in Brownian move- 
ment. On one occasion, when an antherozoid that had become free was 
under observation, this sphere was seen to burst. There can be no question, 
I think, that the sphere in question is the limosphere, still embedded in the 
cytoplasmic mass that adheres to the posterior end of the antherozoid. In 
the case of fixed and stained antherozoids (Figs, 56-9), the cytoplasmic 
remnants include various darkly staining bodies, among which it is not 
usually easy to distinguish the limosphere. Apparently, in the course of 
the processes concerned in making the preparations, the limosphere is often 
burst. Attached to the posterior tip of the antherozoid shown in Fig. 57 is 
a body of somewhat irregular shape whose outer portion is darkly stained ; 
this is probably the limosphere, but the identification is not certain. I have 
not observed the final discarding of the cytoplasmic remnants by the moving 
antherozoid ; but there can be little doubt that, as in the case of the 
antherozoids of other bryophytes, this now useless material is thrown off' at 
some time before fertilization occurs. 
The cilia are apparently attached to the body of the antherozoid a short 
distance behind its anterior end. Some of the published figures of Moss 
antherozoids show the cilia diverging from the extreme tip of the body. It 
is possible in any individual case that the cilia remain appressed against the 
body, and therefore indistinguishable from it, for a greater or less distance 
behind their real point of attachment ; but this would hardly happen in 
every instance, and in a careful study of hundreds of antherozoids I have 
not found one which gave an indication of an attachment of the cilia other- 
wise than as shown in Figs. 56- 9. 
From the description which has now been given of the processes 
concerned in spermatogenesis, it appears that the material of which the body 
of the antherozoid is composed has been derived, so far as our present 
methods allow us to determine, entirely from the blepharoplast and the 
