ATMOSPHERE IN RELATION TO PUTREFACTION AND INFECTION. 
It caused me to turn again to my tubes, seeking further evidence. On the 12th of 
November thirty-six of them were charged, boiled, and hermetically sealed ; on the 13th 
fifty-seven, on the 16th thirty-one, and on the 17th six tubes were similarly treated. 
The entire group of tubes, therefore, numbered one hundred and thirty. I tried 
moreover to multiply the chances of spontaneous generation by making the infusions 
of the most diverse materials. The following Table gives the names of the substances 
operated on, the number of tubes sealed, and the date of sealing : — 
Fowl 
6 tubes. 
November 12 th. 
Mutton . . . . 
6 
33 
33 
Wild Duck . . . 
6 
33 
33 
Beef . . . . 
6 
33 
Herring .... 
6 
33 
33 
Haddock .... 
6 
33 
„ 
Mullet .... 
6 
33 
November 13 th. 
Codfish .... 
. 
6 
33 
33 
Pheasant .... 
6 
Heart .... 
6 
33 
Rabbit .... 
6 
33 
33 
Hare 
6 
33 
Snipe 
6 
33 
33 
Partridge . . 
. 
6 
„ 
33 
Plover .... 
. 
5 
33 
33 
Liver 
4 
„ 
Tongue of Sheep . 
6 
33 
November 16th. 
Brains of Sheep 
3 
33 
33 
Sweetbread . . . 
6 
33 
33 
Humour of Ox-eye 
(undiluted) 
2 
33 
„ 
Lens of Ox-eye 
3 
„ 
„ 
Lungs of Sheep 
5 
33 
„ 
Tripe 
6 
33 
33 
Sole 
6 
33 
November 17 th. 
The tubes were immersed in groups of six at a time in an oil-bath, boiled for three 
minutes, and then sealed. 
More than one hundred of these flasks were sensibly transparent and free from tur- 
bidity at the outset, and they remain so to the present hour. In some cases, 
however, it was not possible to wholly remove turbidity by filtration. I have already 
referred to the opalescence of oyster-infusion, which has invariably appeared whenever 
oyster has been digested. A still more pronounced case of the kind is furnished by 
an infusion of the crystalline lens of the ox. Nothing hitherto encountered imitates 
