x 5 
Embryo in Lilaea subulata , II. B. K. 
genous cell, one of which by its subsequent growth destroys 
the others, and becomes the embryo-sac. In the case 
figured the lower cell already shows signs of disintegration, 
while in the upper one it is difficult to say whether we have 
the young embryo-sac showing the first nuclear division, or 
what seems more likely, from a comparison with other more 
advanced stages, the second division of the sporogenous.cell, 
with a suppression of the cell-wall. In other cases where the 
young embryo-sac was found with two nuclei, it was much 
larger, and there were the remains of one, and in some cases 
of two sporogenous cells above it. Whether in any instances 
there are four complete sporogenous cells formed can only be 
determined by further investigations. 
After it is once formed, the growth of the embryo-sac 
proceeds rapidly and the other sporogenous cells are destroyed. 
The youngest stages at which the embryo-sac could certainly 
be identified, already showed two nuclei. The cytoplasm did 
not fill the cell, but there was a large ventral vacuole. Near 
each end was a conspicuous but not very large nucleus, 
surrounded by a mass of granular protoplasm. The actual 
divisions of these nuclei were not seen, but there is no reason 
to suppose that they differ from other similar ones. As 
usual the four nuclei derived from each occupy either end of 
the embryo-sac. Those of the upper end are perhaps a little 
larger than those of the antipodal region, but the difference 
is very slight, and perhaps not constant. The nuclei are 
usually distinct with a single large nucleolus. The granular 
cytoplasm is now confined to a very thin layer at the sides 
of the embryo-sac, but is more abundant at the ends where 
the nuclei are situated. The remains of the sister-cell (or 
cells ?) of the embryo-sac are still evident as a structureless 
mass lying above it (Figs. 24, 26). 
There now begins the differentiation of the antipodal cells 
and egg-apparatus. The former, which are later very con- 
spicuous, become invested with evident membranes, probably 
of cellulose, while the two synergidae and the egg soon 
become easily recognizable at the upper end. These are 
