8 1 
C tit l evict multifida ( Grev .). 
was so apparent that the question of the non-sexuality of 
Cutleria was never in doubt. 
The converse was however asserted by Reinke 1 in carrying 
out his researches at the Naples Station in 1875-76. He 
confirmed Crouan’s observations on the peculiar pseudo-tissue 
formation of the antherozoids, but attributed to it no real 
germinative significance, since in all his experiments, anthero- 
zoids and oospheres, isolated from each other, constantly 
underwent no further development. On the other hand, 
in vessels containing both male and female plants, germination 
took place freely, and actual fertilization by the antherozoids 
was observed. From these facts he deduced the perfect 
sexuality of C. multifida and the essential importance of 
the antherozoids ; as also that Thuret’s observations must 
have been due to an accidental parthenogensis. It is inter- 
esting to note that he gives male and female plants as 
occurring in the Bay of Naples in the ratio of three male 
to two female. 
Similarly Falkenberg 2 , in 1878, described male and female 
plants as being about equally abundant in the Bay of Naples, 
and carrying out his experiments with great care in obtaining 
pure cultures of emitted oospheres and antherozoids, he fully 
confirmed Reinke’s results. Moreover, as his cultures were 
free from extraneous growths of Diatoms, &c., which had 
ultimately induced pathological conditions in Reinke’s cul- 
tures, Falkenberg succeeded in developing the germinated 
embryos to a considerable size. In all his experiments, 
antherozoids became immotile and useless in twenty-four 
hours, and then died ; oospheres retained the capacity for 
fertilization for four or five days, but never commenced 
segmentation; fertilized oospheres germinated directly and 
rapidly; while unfertilized oospheres never got beyond the 
formation of a thin cell-membrane. 
Further, Janczewski 3 , at Antibes in 1883, showed in the case 
of C. adspersa , which is also a Mediterranean spring-plant, 
1 Nova Acta der K. L. C. Deutsch. Akad. xl. 1878. 
2 Loc. cit. 3 Ann. Sci. Nat. vi. 16. p. 210. 
G 
