NOTES. 
CORRELATION OP GROWTH UNDER THE INFLUENCE 
OF INJURIES. — In the paper on this subject which appeared in 
the Annals of Botany, Vol. xi, No. XLIV, December, 1897, reference 
was made on p. 513 to Laurent’s valuable paper, Etudes sur la 
Turgescence chez le Phycomyces, but by an oversight the name 
of the author was omitted. 
C. O. TOWNSEND. 
GELATINE AS A FIXATIVE. — Microtome-sections passing 
through embryonic and parenchymatous tissues embedded in paraffin 
are sufficiently fixed to the microscope-slide, for staining purposes, by 
their own simple adhesion to the glass. This, however, is not the 
case when the section comprises a large proportion of woody tissue. 
For such preparations, collodion, agar-agar, and albumen have been 
recommended as fixatives. The first of these, so far as my experience 
extends, is the most certain. But it has the disadvantage that with its 
use the paraffin-section cannot be floated out on water on the slip 
and caused to flatten out by gentle warmth. The same objection 
applies to albumen \ and in addition, I have found it to be very easily 
coloured by stains (especially the blue dyes) which are often essential 
to use in microscopic work. I have no experience with agar-agar, 
but Zimmermann states that it becomes dyed with haematoxylin — 
one of the most important stains, and that the sections often come 
loose from the glass during the staining and washing manipulations. 
This latter objection, perhaps the most vexatious of all, applies, to 
some extent, to albumen also. 
Recently I have used as a fixative a dilute solution of gelatine in 
a watery solution of bichromate of potash. The solution should be 
quite fluid at io°C. In use the ribbon of paraffin-sections is laid 
on a drop of this solution on the slide. Wrinkles in the sections may 
1 I used the preparation given by Zimmermann, Bot. Mikrotech. 
