Ward . — Some Thafyes Bacteria. 297 
Carrot at 25 0 gave good results. In twenty-four hours 
a rapidly-spreading gum-like layer formed, and extended all 
over as a wet, thin, watery layer in forty-eight hours. No 
change on the fourth day, and matters were the same at the 
end of a week. In fifteen days the tubes were discarded — no 
further growth. 
Turnip at 25 0 gave no certain results in four days, and even 
after a week no growth was observable. Kept for three 
weeks — no further results. 
Broth. No growth at 35 0 in three days. The liquid 
remains perfectly clear. At 25 0 C., however, the broth is 
turbid in twenty-four hours and with a dense precipitate, 
which is white in three days. Even after a fortnight the 
liquid is still densely turbid, and a copious flocculent pre- 
cipitate has fallen. 
Glucose at 25 0 showed no trace of activity in three days. 
A tube put in at 30°, when the temperature was falling to 25°, 
gave a slight turbidity in three days, and traces of white 
precipitate, but no fermentation visible ; this remained the 
same on the ninth day. In other cases no results were 
obtained in two or three weeks at 25° C. 
Milk at 25°C. No change to third day, but in fifteen days 
a thick custard is formed, and the tube can be upturned. In 
eighteen days the casein falls. The reaction is acid. No 
signs of solution in five weeks at 25 0 C. 
Urine at 22 0 gave a slight turbidity in five days, with 
traces of a ring, but no signs of further growth. 
P athogenicity . This form was kindly examined for me by 
Dr. Lazarus Barlow and gave pathogenic results. A guinea- 
pig inoculated in the peritoneal cavity with about 10 c.c. 
of a four days’ old beef-broth-culture died in twenty-three 
hours. 
On examining Dr. Lazarus Barlow’s preparations, I found 
them exactly to type. That from the peritoneal fluid showed 
the capsules, faintly but distinctly, but in the others they 
were almost invisible. It should be noted that no information 
had been given when the tubes were handed on, and so no 
