3 H Ward. — Some Thames Bacteria . 
ring. In three weeks still turbid, white ring, and copious 
yellowish or buff deposit. 
Milk at 25 0 shows no change in fourteen days. In three 
weeks it is just acid, but no apparent alteration. 
Glucose at 25 0 . A slight white deposit in three days. In 
three weeks greasy flecks above and a fairly abundant 
yellowish-white deposit. 
This form is not pathogenic to guinea-pigs, according to 
Professor Kanthack. 
It was easily revived from an Agar-tube which had laid 
quiescent from May to June in the following year — i. e. 
thirteen months. It came up very pale and weak at first, 
but soon recovered all its normal characters as described. 
From the sum of the characters, including the results of 
microscopic cultures below, this form presents resemblances 
to B . diphtheriae which cannot be neglected, but it is not 
a Bacillus. 
When I came to make micro-cultures of this organism in 
hanging-drops of gelatine and of broth, some unexpected 
results were obtained of considerable interest and importance. 
The following examples will illustrate this : — 
A gelatine drop-culture twelve hours old had a rodlet 4x1 /x 
at 8 a.m. (t. = 2i°C.), which was fixed and observed under 
the Zeiss E as shown in Fig. 6 ( a-k ). At 9.30 the much 
longer and sharply bent rod was behaving very curiously for 
a Schizomycete, for it appeared to be putting out a branch at 
right angles from its lower segment (Fig. 6 c). 
At 10.30 the much diluted gelatine was nearly fluid and an 
end-segment had broken off to the right and floated somewhat 
to the middle of the parent rod and there divided. The 
further course of the formation of the colony is visible in the 
drawings ( d-h ). At 4.40 p.m. a circular colony 24 /x in 
diameter had been formed (i ) : at 8 p.m. this was 32 ^ in 
diameter (/). Next morning at 9 o’clock it measured 75 /x 
across (fc ) 1 , and by noon it was 90 /x in diameter and quite 
typical. 
1 Sketched under a lower power. 
