512 Wager . — The N tic lens of the Yeast-Plant. 
a very prolonged stay in the solution is necessary in order to 
obtain a good differentiation. The preparations may be 
decolourized more quickly and effectively perhaps in alum 
solution. 
Safranin. A solution in water to which a 3*5% solution 
of aniline in water has been added may be used. The 
preparations remain in this for two or three hours ; they are 
then washed in water, well washed in alcohol and acid 
alcohol, cleared in xylol and mounted in balsam. The nuclear 
body stains but slightly, the same as the protoplasm, but in 
good preparations the vacuole and its contents are coloured 
bright red. 
Gentian-violet . A solution of this in aniline-water is very 
useful. Preparations should be stained for about half an hour; 
then washed in water and thoroughly washed out in 7o°/ 0 
alcohol, and finally mounted in balsam. In good preparations 
the nucleus stains pale reddish blue, the granules and 
vacuolar contents a deeper reddish colour. It is a very 
intense stain, and when carefully used gives good results. 
The combination of this stain with safranin and orange has 
not in my hands been productive of good results. 
Fuchsin and methylene-blue. Stain first in carbol-fuchsin, 
then wash out in water and dilute alcohol, or very dilute 
solution of sulphuric acid, and subsequently stain in a dilute 
aqueous solution of methylene-blue. The nuclear body and 
spores are red, the protoplasm blue. This is not a very good 
combination for the study of Yeast-cells. 
Carbol-fuchsin . Janssens and Leblanc give this as a good 
stain ; but I have found that the combination of fuchsin and 
methyl-green, or fuchsin and methylene-blue, is far more 
effective. By itself the fuchsin stains somewhat diffusely. It 
is however an excellent stain for ripe spores when used hot, 
as in the staining of spores of Bacteria : preparations should 
be mounted in balsam. 
Carmine and Nigrosin . This is used according to the 
method given by Hartog (’95) and gives fairly good results. 
It is necessary, however, to be very careful to wash out the 
