Wager. — The Nucleus of the Yeast-P lant. 519 
preserved in methylated spirit ; and most of the ordinary 
reagents used for fixing render it as a rule more or less 
visible x . 
In fresh Yeast it can in some cases be made out by careful 
examination under the one-twelfth inch objective, especially 
in compressed Yeast. As seen in the fresh condition, it is 
a pale slightly refringent spherical body. Its presence is 
often masked in compressed Yeast by the granules around it, 
but its position may be indicated by a slight flattening or 
indentation of the vacuole on that side on which it is placed. 
The relation of Hieronymus’ granules to the nuclear body 
is interesting. They may be easily observed if ordinary 
compressed Yeast be placed in very dilute sugar-solution and 
examined with a high power. Nearly all the cells will then 
be found to contain them. There may be only a few present, 
as in Fig. 33, or many, as in Figs. 34-40. In some cases 
they are grouped closely around the nucleus, as if connected 
in some way with it (Fig. 34), probably for purposes of 
nutrition. Sometimes the granules are found only on one 
side of it, sometimes on two sides or all round (Figs. 34 and 
36), except in the region of the cell-wall and the vacuole. In 
addition to the granules around the nucleus we find a few or 
many in the protoplasm around the vacuole. In other cases 
the granules are not found grouped in this way round the 
nuclear body, but are distributed more or less regularly 
through the cell (Figs. 38, 39). In cells which had been kept 
in dilute sugar-solution for some hours, the granules were 
more commonly found grouped around the nucleus. After 
about twelve hours in a warm place in dilute sugar-solution, 
the granules increase in number, the protoplasm becomes 
vacuolar, and the nucleus takes up a position more in the 
centre of the cell, where it is surrounded by the granules on 
all sides (Figs. 39, 40). Sometimes the granules seem to 
1 Cells of S. Cerevisiae placed in a solution of alkanin for twenty-four hours or 
longer also show the nuclear body quite clearly, stained light red. The same 
body also gives, in cells hardened in alcohol, a definite reaction for phosphorus 
when treated according to the method described by Macallum (’98). 
N n 
