522 Wager . — The Nucleus of the Yeast- Plant. 
of fermentation, and stained according to the method already 
described, in methyl-green and eosin, for a few seconds, 
washed in water and examined in dilute glycerin, showed 
the following structure in different cases : — 
1. A small vacuole (nuclear vacuole) containing granules 
and a delicate network stained green or blue, and a few 
granules which remain unstained ; a layer of granular 
protoplasm stained pink, and a nuclear body in close 
contact with the vacuole, but never inside it, also stained 
pink or reddish blue (Figs. 1-4). 
2. Small cells stained intense green all through, with 
a nuclear body (green), vacuole and granular contents (green), 
and homogeneous protoplasm (green) (Fig. 31). 
3. Small cells with numerous vacuoles in a homogeneous 
protoplasm, and a nuclear body, all stained intense green, 
or in some cases with vacuoles and nuclear body green, 
protoplasm blue. Sometimes the nuclear body was found 
in the midst of the vacuoles, sometimes on one side of them 
(Figs. 28-30). 
4. Cells with nuclear body blue, vacuole and contents blue, 
protoplasm pink. 
5. Small cells with apparently no nuclear body, but with 
protoplasm and vacuole stained intense green. The absence 
of a nuclear body is only apparent however, for on carefully 
washing out the stain it is brought into view, and in methyl- 
green and fuchsin by sufficient washing out it is always 
visible, stained red. 
6. Cells in which the vacuole is surrounded more or less 
completely by granules, which are stained blue. The vacuole 
contains very little stainable substance in most cases 
(Fig. 10). 
7. Cells here and there with nuclear body blue, a vacuole 
present but not well marked, pink protoplasm, and a number 
of granules (blue) scattered through the protoplasm. 
8. Cells in which a vacuole is not visible, but in its place 
a more or less regular granular network in contact with the 
nuclear body (Fig. 9). 
