Wager . — The Nucleus of the Yeast-Plant. 523 
Staining in methyl-green and fuchsin for a few seconds 
produces the same effect, but the nuclear body stains red, 
and the vacuole and its contents are not so clearly differ- 
entiated. Nevertheless, with careful staining good results are 
obtained. In successful preparations the nuclear body is 
bright red, the vacuole and its network deep blue, the proto- 
plasm faintly stained blue. In some respects this is a more 
useful combination than methyl-green and eosin. 
I11 aniline- water-safranin, the vacuolar network stains bright 
red, the nuclear body and the protoplasm light red. 
In Delafield’s haematoxylin — a solution which had been 
kept a long time — the nuclear body stains light red, the 
vacuolar network and granules deeper red. By the method 
of Heidenhain the nuclear body stains deep blue or black, the 
vacuole and contents lighter blue or black, the protoplasm 
remaining colourless or only faintly stained. 
The nuclear body is always in close contact with the 
vacuole, and appears to be very intimately connected with 
it. Even when from whatever cause any contraction of the 
vacuole takes place, the nuclear body always remains in close 
contact with it, and one is never able to see any divisions 
between the two (Fig. 27). Granules inside the vacuole are 
often seen in contact with the nuclear body, and in some 
cases appear as if about to become absorbed into it. It 
seems likely that as the cells become older the contents 
of the vacuole may in part become absorbed into the nuclear 
body. 
The appearance of the vacuole varies in different cells. In 
some cells it is large and contains very little stainable matter ; 
in other cells it is small and often contains a dense mass 
of stainable substance. The stainable substance in the 
vacuoles is partly in the form of a network, partly in the 
form of granules. In some cells the network is distinctly 
granular, in others it consists of very fine, delicate threads. 
In some vacuoles there is sometimes a large, sometimes a 
small, central portion which stains deeply and is surrounded 
by delicate threads connecting it to the membrane of the 
