526 Wager . — The Nucleus of the Yeast- PI ant. 
nuclear apparatus is in the main similar to that of 5 . Cerevisiae 
(Figs. 41-53). The nuclear vacuole seems to persist for 
a longer time however, as it does in compressed Yeast. The 
nuclear body is in close contact with the vacuole as a rule, 
but occasionally it may be separated some distance from it 
(Fig. 50). One often finds that a definite generally curved 
row of granules extends from the nuclear vacuole through the 
protoplasm to one or both ends of the cell (Figs. 43, 49, 50). 
In addition to the chromatin-vacuole, there are one or two 
large vacuoles present normally in a cell in the resting 
condition. As in 6*. Cerevisiae , there may be two or more 
small vacuoles present in young cells in place of one (Fig. 
44, 48). 
Origin of the Vacuole. 
Young cells often contain numerous vacuoles surrounding 
the nuclear body (Figs. 28, 29, 30, 32). Some of these 
vacuoles are very small and are nearly filled up completely 
by a granule which stains an intense green, and thus appears 
to be of the nature of chromatin (Fig. 28). Under a low 
power these small vacuoles present the appearance of granules 
merely, and would be easily mistaken for the ordinary granules 
of a Yeast-cell ; but under a high power their vacuolar nature 
can be easily made out in well -stained specimens. It is 
difficult to escape the conclusion that the vacuoles arise 
in some way in connexion with the granules. As the cell 
grows the vacuoles gradually fuse together to form the single 
vacuole in close contact with the nuclear body. 
I have observed the same phenomenon in young Fungus- 
hyphae, probably Mncor , which constantly occur among the 
Yeast-cells in my cultures. In these hyphae the nuclei 
appear to be homogeneous in structure, and indeed stain in 
precisely the same manner as the nuclei of the Yeast-cells near 
them. In all the younger hyphae large vacuoles can be seen 
containing a substance staining green just as occurs in Yeast- 
cells, and these large vacuoles arise by the fusion of numerous 
smaller ones. 
