Wager . — The Nucleus of the Yeast-Plant . 537 
into the nucleolus, which then divides by elongation and con- 
striction into two. • During the division deeply stained granules 
(chromosomes ?) appear surrounded by a less deeply stained 
substance, which remains for a time connecting the two daughter- 
nucleoli together. This may perhaps indicate a simple inter- 
mediate stage of karyokinesis. 
9. Subsequent divisions take place resulting in the formation 
of four (sometimes more) nucleoli. Each nucleolus becomes 
surrounded by protoplasm and a delicate membrane, and thus 
the spores are formed standing free in the remainder of the 
protoplasm. 
10. The spores are at first very small, but they soon increase 
in size ; the surrounding protoplasm becomes used up ; the 
spore- membranes increase in thickness until at last in the 
mature condition they completely fill the mother-cell. 
11. In S. Ludwigii and vS\ pastorianus the structure of the 
nuclear apparatus is similar to that in vS. Cerevisiae , and its 
division during the process of budding appears to be also the 
same. 
O O 2 
