540 Wager . — The Nucleus of the Yeast-Plant. 
EXPLANATION OF FIGURES IN PLATES 
XXIX AND XXX. 
Illustrating Mr. Wager’s Paper on the Nucleus of the Yeast-Plant. 
All the figures have been drawn freehand, with the aid of Zeiss’s apochromatic 
2 mm. aperture i- 4, and oculars 8 ( x iooo), 12 (x 1500), and 18 ( x 2250). In 
most cases the outlines of the cells, &c., have been drawn with the aid of the 
camera lucida. Figs. 1-3, 5-21, 28-32, and 41-53 have been drawn from 
preparations stained in methyl-green and eosin; Fig. 4, safranin ; Figs. 22-27, 
methyl-green and fuchsin ; Figs. 33-40 from living cells ; Figs. 54-57, 60, 62, and 
63* gentian-violet ; Figs. 58, 59, 61, 64, 65, 68, and 72-78, carmine and nigrosin ; 
Figs. 66, 67, and 69-71, Heidenhain’s haematoxylin. 
Saccharomyces Cerevisiae. 
Figs. 1-5, 7-12, 14-18, and 27-32, after three hours in Pasteur’s solution. 
Fig. 1. Cell showing nuclear body and vacuole with network and one deeply 
stained granule. 
Fig. 2. Ditto showing three deeply stained granules in the vacuole. 
Fig. 3. Ditto showing a vacuole not much larger than the nuclear body. 
Fig. 4. Cell containing a vacuole which shows the nuclear-like network very 
clearly. 
Fig 5. Cell with vacuole full of deeply stained substance, partly enclosing the 
nucleolus. 
Fig. 6. Cell of compressed Yeast — the vacuole contains two deeply stained 
granules and delicate threads. Both nuclear body and vacuole are to some 
extent surrounded by unstained granules — Hieronymus’ granules. Two hours 
in sugar-solution. 
Fig. 7. Cell showing nucleolus as seen through a vacuole. The appearance is 
presented of a nucleus with nuclear membrane, &c. 
Fig. 8. Cell showing nucleolus, in part surrounded by a dense mass of 
granules. 
Fig. 9. Shows the nucleolus in contact with what appears to be a very definite 
chromatin-network. Whether this is contained in a vacuole or not, could not be 
made out clearly. 
Fig. 10. Shows the position of the nucleolus at the time when the cell begins to 
bud. The vacuole in this case contains very little, if any, stainable substance, but 
is surrounded by deeply stained granules. 
Fig, 11. The vacuole, with granular contents, is making its way into the young 
bud. The nucleolus still retains its position on the opposite side of the cell. 
Fig. 12. The nucleolus has made its way to the opening between the bud and 
the parent- cell. 
Fig. 13. Ditto. The vacuole in this case is surrounded by deeply stained 
granules. Seventy-two hours in Pasteur’s solution. 
Fig. 14. The nucleolus puts out a projection into the neck of the budding cell. 
