Wager. — The Nucleus of the Yeast-Plant. 541 
Fig. 15. Ditto, but a slightly later stage. The vacuolar contents are very 
abundant. 
Fig. 16. Slightly later stage than Fig. 15, just previous to the complete division 
of the nucleolus. The vacuole is small and irregular in shape. 
Fig. 17. The same stage as Fig. 16, but the vacuole has nearly disappeared, and 
in its place a deeply stained mass of granules nearly equally divided between the 
two cells. 
Fig. 18. Complete separation of the newly formed nucleoli to opposite ends of 
their respective cells. A granular thread is shown drawn out between the two from 
the granular network. 
Figs. 19-24. After seventy- two hours in Pasteur’s solution. 
Fig. 19. Shows the nucleolus beginning to divide by constriction in the parent- 
cell. 
Fig. 20. Later stage than Fig. 19, the division is completed even before the 
vacuole begins to divide. 
Fig. 21. Division of nucleolus in the parent-cell. A small vacuole only is 
present but there are a number of deeply stained granules, which are separated 
into two equal groups with a granular thread drawn out between them. 
Fig. 22. Shows the glycogen-vacuole beginning to form. The chromatin-vacuole 
is still present with one deeply stained granule, and near the nucleolus numerous 
deeply stained granules are to be seen. 
Fig. 23. Later stage — the large vacuole is the glycogen-vacuole. 
Fig. 24. The nucleolus, chromatin-vacuole, and granules restricted to the wall of 
the cell by the glycogen-vacuole. 
Fig. 25. S. Cerevisiac, Hansen I, shows nucleolus and granular network and 
large glycogen-vacuole. 
Fig. 26. S. Cerevisiae, Hansen I, shows a nucleolus lying on the wall of the 
cell surrounded by a lightly stained vacuolar protoplasm, containing a few deeply 
stained granules. The remainder of the thin layer of protoplasm lining the cell is 
granular. 
Fig. 27. Cell showing the gradual formation of the glycogen-vacuole, and the 
contraction of the chromatin-vacuole. Note the close attachment of the latter to 
the nucleolus. The protoplasm contains numerous deeply stained granules. 
Fig. 28. Young cell with numerous small vacuoles, each enclosing a deeply 
stained granule, and some granules with a vacuole apparently just forming around 
each. The whole of the cell contents stain deeply. 
Fig. 29. Young cell with three vacuoles, each containing a deeply stained 
granule. The cell contents stain deeply. The nucleolus is only visible after very 
careful staining. 
Fig. 30. Ditto, with four vacuoles. 
Fig. 31. Young cell with one vacuole, containing a deeply stained granule and 
delicate radiating threads. Near it a nucleolus only visible with difficulty. 
Fig. 32. Young cell with nucleolus in the midst of a peripheral ring of granules. 
The whole cell is pervaded by a deeply stainable substance. 
Figs. 33-40. Compressed Yeast examined in the living condition. The black 
granules represent the highly refractive granules described by Hieronymus. 
Fig* 33 * Half an hour after being placed in 5 % sugar- solution. The nucleolus 
is visible, and is indicated by a slight depression in the vacuole. Few refractive 
granules present. 
