542 Wager . — The Nucleus of the Yeast-Plant. 
Fig. 34. The nucleolus is surrounded by the refractive granules, which are now 
more numerous. Two moving granules in the vacuole. Two hours in sugar- 
solution. 
Fig. 35. The bright granules more numerous. Two vacuoles present and 
a nucleolus. Two hours in sugar-solution. 
Fig. 36. Shows three pairs of granules and small groups on each side of the 
nucleolus. Two hours in sugar-solution. 
Fig. 37. Numerous vacuoles appear, as a preliminary to spore-formation. 
Three hours in sugar-solution. 
Fig. 38. Later stage — the vacuoles are more numerous, the bright granules 
surround the vacuoles. Three hours in sugar-solution. 
Fig. 39. The vacuoles disappear. The protoplasm, as shown by means of 
reagents, exhibits a foam-structure at this stage. Twenty-four hours in sugar- 
solution. 
Fig. 40. Two groups of granules on opposite sides of the nucleolus in a hyaline 
protoplasm. Twenty-four hours in sugar-solution. 
S. Ludwigii. 
Fig. 41. Cell showing chromatin-containing vacuole and nucleolus. 
Fig. 42. Ditto. Two lines of granules run from one end of the cell to the 
nuclear vacuole. The figure shows the nucleolus as seen above the vacuole, not 
inside it. 
Fig. 43. Young bud just forming. Nucleolus beginning to divide. One row of 
granules stretching from one end of the cell to the nuclear vacuole. 
Fig. 44. Cell showing two vacuoles and a nucleolus between them. 
S. pastorianus. 
Fig. 45. Cell showing two large normal vacuoles, and in the strand of proto- 
plasm across the middle a nuclear vacuole and a nucleolus on one side. 
Fig. 46. Shows the nucleolus as seen from above. On focussing down the 
vacuole could be seen. 
Fig. 47. Cell showing nucleolus as seen through the vacuole. 
Fig. 48. Cell with nucleolus and three small chromatin-vacuoles. 
Fig. 49. Cell showing nucleolus at one end surrounded by granules which are 
connected to a small vacuole at the other end by a deeply stained row of 
granules. 
Fig. 50. Nucleolus in the middle of the cell in contact with a curving line 
of granules running from a chromatin-vacuole at one end to the other end of 
the cell. 
Fig. 51. Cell with young bud. The nucleolus is in contact with a vacuole 
containing a deeply stained granule and network. 
Fig. 52. Later stage than Fig. 51 ; the nucleolus is beginning to divide. 
Fig. 53- Case of budding in which the nucleolus is about to divide in the neck 
joining the two cells. The daughter-cell contains a chromatin-vacuole in close 
contact with its share of the nucleolus. 
Spore-formation. 
Fig. 54. Cell just at commencement of spore-formation — protoplasm reddish 
blue, nucleolus light blue. 
