Wager . — -The Nucleus of the Yeast- Plant. 543 
Fig. 55. Cell in which the deeply stainable substance is becoming concentrated 
in a central mass of protoplasm. The nucleolus stains light blue as before, but 
a little more deeply. 
Fig. 56. Later stage. The nucleolus shows a deeply stained granular substance 
inside or in close contact with it. 
Fig. 57. The deeply stained granular mass inside or in contact with the nucleolus 
has increased in size. The deeply stained central portion of the protoplasm is 
surrounded by granules. 
Fig. 58. Later stage. The deeply stained mass of protoplasm is smaller. 
Fig. 59. Still later stage. The deeply stained nucleolus is now the most 
prominent structure. 
Fig. 60. Commencement of division. The nucleolus and its deeply stained 
mass begin to elongate. 
Fig. 61. The deeply stained granular mass at a later stage in the process of 
elongation. 
Fig. 62. The row of deeply stained granules stretching across the cell, surrounded 
by a faintly stained substance. 
Fig. 63. Shows the gradual accumulation of the granular substance at both ends 
to form the daughter-nucleoli. 
Fig. 64. Complete separation has now taken place. 
Fig. 65. Later stage in the division ; the two daughter-nucleoli are still connected 
together by a less deeply stained substance. 
Fig. 66. Division- stage, as shown in a preparation stained with haematoxylin, 
Heidenhain’s method. One portion of the protoplasm is still shown more deeply 
stained than the other, with a distinct line of separation between them. 
Fig. 67. Same method of preparation. The division is transverse. 
Fig. 68. The two groups of deeply stained granules completely separated, each 
surrounded by a less deeply stained substance with a faintly stained granular thread 
drawn out between them. 
Fig. 69. Division into four. 
Fig. 70. Slightly later stage than Fig. 68. 
Fig. 71. Shows a cell in which division is taking place into eight nucleoli 
instead of four. 
Fig. 72. Four groups of deeply stained granules, still connected together by less 
deeply stained substance. The division apparently has taken place at one end of 
the cell. 
Fig. 73. Stage similar to Fig. 71, but the groups of granules are more irregularly 
distributed at one end of the cell. 
Fig. 74. The four products of the previous two divisions are now lying in 
a slightly more deeply stained portion of the protoplasm at one end of the cell. 
Fig. 75. Later stage than Fig. 74. The spores beginning to separate out. 
Fig. 76. The spores now visible, each with a distinct outline due to the presence 
of a thin membrane. 
Fig. 77. Later stage. The membrane is more distinct. 
Fig. 78. Same stage as Fig. 77, but five spores shown, two smaller than the 
others. From observations made recently I am inclined to think that these two 
fuse together to form one. 
