Notes 
567 
mycelium at the growing-points of the flower shoots, and in such 
sections the mycelium, at first intercellular, could readily be found 
becoming intracellular and of much greater diameter. Branches 
became very numerous, and in the hyphae and branches spores were 
formed. Towards the central parts spore-clusters were too dense for 
examination, but nearer the epidermis the branching and arrangement 
of the sporogenous hjphae could more easily be made out; and the 
teasing of the lateral flowers of each notch of the rachis was often 
more successful than if the central — and only flower of the ordinary 
ear— were taken. Sections were mounted in water, and some in 
1 per cent. KOH, and it is but fair to say that such treatment has 
failed to show any septation of the hyphae as a preliminary to spore- 
formation. Material for microtome-sections was prepared as follows : — 
The leaves of a barley-shoot were stripped down so as to expose the 
apparently highest node, and the part an inch or two above this was 
cut off; then by a series of successively lower horizontal cuts the 
youngest leaves were removed until in the space they enclosed the 
tips of the awns or ear were seen ; then a cut was made through 
the node, and the removed ear was placed in Flemming’s or Rath’s 
solution for fixing, the ear thus being, for a very few seconds only, 
between plant and reagent. 
If a smutted ear be removed and kept floating on water, its spores 
continue to develop, and in several cases they matured first in the 
awn. It was by no means uncommon, on teasing out young fruits 
from such an ear, to find that the spores had germinated. 
I have not yet made similar observations for Tilletia as my bunted 
wheat was less forward than my smutted barley, but I am satisfied 
that by this method of working class material for illustrating Bunt 
and Smut may easily be obtained. 
W. G. P. ELLIS, Cambridge. 
STRUCTURE OP THE YEAST- CELL.— A study of the cells 
of Saccharomyces Cerevisiae has led me to the following conclusions, 
part of which merely confirm former researches : (1) A relatively 
large nuclear body exists in each adult cell. (2) Young cells contain 
no such body; a little later the old nuclear body divides, and one* 
of its two daughters wanders through the narrow connecting-channel 
into the young cell. (3) After the division is complete, the two cells 
are still kept together by a mucilaginous neck-shaped pedicel, which 
Q q 
