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VII. New Observations upon the Minute Anatomy of the Papillae of the Frog's Tongue. 
By Lionel S. Beale, M.B., F.B.S . , Fellow of the Royal College of Physicians , 
Professor of Physiology and of General and Morbid Anatomy in King's College , 
London ; Physician to King's College Hospital , &c. 
Received June 16, — Read June 16, 1864. 
In this paper I propose to give the results of some recent investigations upon the minute 
anatomy of the beautiful fungiform papillae of the tongue of the little green tree-frog 
(Hyla arbored). The specimens have been prepared according to the principles laid 
down in former communications. The success I have met with in this and other minute 
anatomical inquiries is, I believe, almost entirely due to the process of investigation 
which I have adopted for some years past, and which enables me to render specimens 
very transparent, and to demonstrate all the tissues in one specimen. By this plan 
sections are obtained so exceedingly thin, without the destruction even of the most 
delicate tissues, that they may be examined under the highest powers which it is possible 
to obtain (-^g- magnifying 1700 linear, and magnifying about 3000 linear). 
The following are among the most recent contributions to the anatomy of the papillae 
of the frog’s tongue : — 
Waller: “Minute structure of the Papillae and Nerves of the Tongue of the Frog 
and Toad,” Philosophical Transactions, 1847. 
Billroth : “ Ueber die Epithelzellen der frosch-zunge, sowie iiber den Bau der 
cylinder-und flimmerepithelien und ihr Verhaltniss zum bindegewebe,” Archiv fur 
Anat. Physiologie, 1858, S. 163. 
Hoyer : “ Mikroskopiche Untersuchungen iiber die zunge des Frosches,” Archiv fur 
Anat. Phys. 1859, S. 488. 
Axel Key: “Ueber d. Endigungen d. Geschmacksnerven in der zunge Frosches,” 
Muller’s Archiv, 1861, S. 329. 
Hartmann : “ Ueber die Endigungsweise der nerven in den Papillae-fungiformes der 
Froschzunge,” Archiv fur Anat. Phys. 1863, S. 634. 
Although the views of Axel Key are supported by schematic figures which do not 
accurately represent the real arrangement of the tissues, they approach much nearer 
to the truth than those of other observers. He describes two kinds of cells at the 
summit of the papilla, epithelial and special cells concerned in taste. I have not 
been able to verify his statements in this particular. He has not demonstrated the 
peculiar network at the summit of the papilla which is seen so distinctly in my speci- 
mens, and his delineations of the prolongation of the axis-cylinder alone, and its divi- 
mdccclxv. 3 p 
