PHYSICAL AND CHEMICAL AGENTS TJPON BLOOD. 
715 
Gas from ox-blood plus chloroform, twenty-four hours’ action, 100 per cent, of atmo- 
spheric air : — 
No. 57. — In 100 parts of air. 
Oxygen . . 
Carbonic acid . 
Nitrogen . . 
1 Q.7Ci 
1< gg|Total oxygen 20-64' 
79-36 
This result proves that chloroform possesses the property of diminishing the power of 
the constituents of the blood to unite with oxygen, and give off carbonic acid. A pre- 
cisely similar result was obtained when the experiment was made on the blood of the 
young animal. 
Perhaps as chloroform is so important an agent I may be pardoned quoting an expe- 
riment performed on the blood of the calf, which proves the correctness of the above 
assertion. 
Equal parts of well-oxygenated freshly-dehbrinated calf’s blood were treated during 
twenty-four hours in receivers in the usual way. One was kept pure, and the other had 
three drops of chloroform added to it (as in the other cases the quantity of blood employed 
was 62 grammes). 
Gas from pure 
spheric air: — 
calf’s blood, twenty-four hours’ action, with 100 per cent, of atmo- 
No. 58. — In 100 parts of air. 
Oxygen . . . 
Carbonic acid . 
Nitrogen . . 
n^lTotal oxygen 18-04 
0*94 J 
81-96 
Gas from calf’s blood plus chloroform, twenty-four hours’ action, with 100 per cent, of 
atmospheric air. Result : — 
No. 59. — In 100 parts of air. 
Oxygen . . . 
Carbonic acid . 
Nitrogen . . 
1 2 . 3 g} Total oxygen 20-93 
79-07 
It is thus seen that chloroform acts in the same manner on the blood of the young as 
on that of the adult animal. 
Oxygen. 
Carbonic acid. 
Nitrogen. 
Total oxygen. 1 
In 100 parts of air from 
Pure ox-blood 
10-42 
5-05 
84-53 
15-47 
Ditto plus chloroform 
18-76 
1-88 
79-36 
20-64 
Pure calf’s blood 
12-10 
5-94 
81-96 
18-04 1 
Ditto plus chloroform 
18-05 
2-88 
79-07 
20-93 
1 
