OF THE BARK OE ERYTHROPHLEUM GUINENSE. 
635 
a property not shown by the watery extract, as is shown in Experiment XIV. This 
difference in the properties of the two extracts does not show itself in the general action 
of the drug on animals ; but the power of the alcoholic extract to prevent the develop- 
ment of Bacteria , while it is without action on them after their development, is interest- 
ing, as substantiating the results of Buchholz’s experiments on this subject with other 
drugs*. 
Three pieces of fresh muscular tissue were placed in bottles on March 22nd. The 
first contained a watery solution of the alcoholic extract of casca, the second a -g oo solu- 
tion of sulphate of quinia, and the third distilled water. On March the 29th the bottles 
were opened ; and while the bottle containing water -was very offensive, and the water 
was crowded with Bacteria , neither the quinine nor casca solutions contained any Bac- 
teria at all. 
The bottle containing the casca solution was again examined on May 14th, and was 
found, as before, quite free from Bacteria. Long before this a thick crust of Penicillium 
had formed on its surface. 
Experiment XIV. 
A piece of fresh cat’s liver was placed in a solution of casca of the same strength as 
that used in the preceding experiment ; but the watery extract was used instead of the 
alcoholic. At the end of two days the liquid was found to be crowded with Bacteria. 
This experiment was afterwards repeated with muscular and other tissues with the 
same result. 
Effect on the Life of Bacteria. 
The effect of the drug on the life of Bacteria , when developed, was tried in Experi- 
ment No. XV. For this purpose an infusion of hay was made, and found to contain 
many rod-shaped Bacteria. To a drop of this infusion a drop of a -yg solution of both 
the alcoholic and the watery extracts of casca was added at different times, and the 
movements of the Bacterians carefully watched under the microscope. They did not, 
however, seem in any way affected by the addition. 
For the sake of comparison a ygg solution of quinia sulphate was added to the hay- 
infusion. The Bacterian movements were found to be instantly stopped. 
Effect on Bed and White Blood-corpuscles. 
In Experiment XVI. the action on the red and colourless blood-corpuscles of the 
newt was investigated. The effects produced by the addition of dilute solution of casca 
to the blood were cessation of amoeboid movements and rounding of the white cor- 
puscles, with an irregular shrinking of the nucleus, and general crenation of the red 
ones. These effects were probably due to the action of the tannic acid contained in the 
extract. 
MDCCCLXXVII. 
Archiv f. exper. Pattiologie u. Pharmakologie, iv. p. 1. 
4 X 
