220 
DR. E. KLEIN ON THE SMALLPOX OE SHEEP. 
in chromic acid and those of the other half hardened in spirit was very striking. In the 
latter case the oedema of the tissue of the corium could not be detected at all, whereas 
in the former the distribution of it and the changes of the elements of the tissue were 
very well preserved. 
Section III.— INVESTIGATION OP THE ORGANISMS CONTAINED IN FRESH LYMPH 
BY CULTIVATION. 
Previous Investigations. — In a paper by Professor Coiin, of Breslau, published in the 
55th volume of Viechow’s ‘ Archiv,’ I find quoted the statements of the more important 
observers who have studied organisms in the fresh lymph of cow-pox and human 
smallpox. 
Kebee (Viechow’s ‘Archiv,’ vol. xlii.) found in fresh lymph peculiar granular corpuscles 
which were perfectly different from pus-corpuscles ; they are about 3-^0 to x5~o °f a li ne 
in diameter, and contain 3 to 20 spherical elongated or hourglass-shaped particles, -g-^o 
to 3^5-0 of a line in diameter. 
After the solution of the cell-wall, those particles having become free, they are distri- 
buted through the lymph in enormous numbers, forming chiefly aggregates of 2 to 4 
and 6 individuals, which are connected by a very delicate intervening substance. They 
divide rapidly into smaller and smaller particles. In old lymph (vaccine tubes) there are 
always present flakes and coagula, which consist of groups of the above-mentioned 
granular corpuscles, free particles and molecules held together by an intervening sub- 
stance. These bodies represent the carriers of the virus. Kebee could not, however, 
determine whether these particles are simply changed nuclei of the cells of the rete 
Malpighii of the epidermis, or whether they are living organisms. 
Halliee and Zuen (Viechow’s ‘ Archiv,’ vols. xli. & xlii.) found in the lymph of cow- 
pox, sheep-pox, and human smallpox a swarming caudate Micrococcus of a conical 
shape endowed with a rotatory movement sometimes in the act of division. 
Besides this they found delicate Leptothrix- filaments ( Micothrix , Itzigsohn), in each 
small chain of which there was a distinct Micrococcus- swarm. 
By cultivation Halliee thought to be able to transform the Micrococcus of the lymph 
into sporidia — further, into Cladosp orium, Sporidesmium , Tilletia , Monilia , Pleospora 
herb arum, Oidium, Eurotium , Aspergillus, Stemphylium, Ustilago, Torula, and other 
forms, all of which he regarded as different stages of development of the Micrococcus 
of variola. 
As the sporids originating from Micrococcus of sheep-pox develop,, according to 
Halliee, in the air to a Cladosporium, which is identical with one of the forms of Pleo- 
spora recognized by Tulasne as the conidium-bearing form, and as this latter, a parasi- 
tical fungus living on Lolium perenne, is contained in spoiled hay, the inference which 
may be drawn from this is obvious according to Halliee, viz. that spoiled hay is the 
source of infection of sheep-pox. 
Chauveau deduced the presence of organic particles being the carriers of the contagion 
