224 
DE. E. KLEIN ON THE SMALLPOX OE SHEEP. 
Spheres in the act of transverse division are very often met with. 
It is worth noticing that the few rod-like Bacteria mentioned above as being present 
in the fresh preparation disappeared completely after the preparation had been kept in 
the incubator twenty-four to forty-eight hours. 
A drop of lymph was obtained on March 24 from a pustule of an animal which had 
been infected March 10 (see experiment 3), and was used for a microscopic preparation 
as in the former case, without, however, being diluted with saline solution. When 
examined fresh, it showed, besides large numbers of granular pus-corpuscles and 
coloured blood-corpuscles, numerous small highly refractive granules, isolated and in 
couples, exhibiting molecular movement. . The preparation was placed in the incubator 
and kept at a constant temperature of 32° C. for twenty-seven hours, after which time 
when examined it showed the following structures : — 
(a) Besides intact granular pus-corpuscles there were numerous pus-corpuscles the 
substance of which had become swollen and transparent ; these contained two to six 
spherical homogeneous, not very highly refractive, bodies, about half the size of a coloured 
blood-corpuscle, or even less. Some pus-corpuscles containing these bodies were seen 
to be in the state of becoming disintegrated, and thus those spherical bodies becoming 
freed. That they are not nuclei of the pus-corpuscles is shown by the fact that they 
become the more distinct the more the matrix of the pus-corpuscle becomes swollen 
and disintegrated. They are most distinct when they have become freed from a cor- 
puscle. Besides they have a slightly greenish colour and are homogeneous ; whereas it is 
well known that when pus-corpuscles swell, also their nuclei become swollen, and have 
then the appearance of vesicles bordered by a thin membrane. Similar spherical bodies 
are found in the surrounding medium in great numbers ; they are either isolated or in 
couples ; they are generally spherical ; occasionally they are oblong, and possess a more 
or less deep constriction in the middle part. 
(b) From these forms one can trace others, which possess one or two small dark 
granules ; in the latter instance the corpuscle is generally somewhat elongated, and the 
grannules are situated at its pole. 
From these, again, we come to other forms, which consist of two granules (dumb-bell) 
surrounded by a very thin pale envelope, and, finally, dumb-bells in which there is just 
a trace of the envelope to he seen under a very high power. I refer the reader to l in 
fig. 3, Plate 29, in which most of the forms just mentioned are represented. 
From this we are justified in saying that there exist spherical bodies, either enclosed 
in pus-corpuscles or freely suspended in the medium, which are not nuclei; they are 
isolated or in couples (transverse division), of a slightly greenish colour, homogeneous, 
and pretty nearly of the same size. 
It may be further stated that these spheres become transparent, while in them gra- 
nules, i. e. highly refractive minute spheroids ( Micrococci ), make their appearance ; these 
multiply by the act of transverse division (dumb-bells), and the matrix now represents 
a transparent more or less distinct envelope or connecting substance of the dumb-bells. 
