DE. E. KLEIN ON THE SMALLPOX OF SHEEP. 
225 
( c ) That there is going on in these dumb-bells an immensely rapid proliferation by 
transverse division is proved by the really astounding number of Torula-Wke chains 
(necklaces), most of them consisting of 4, many of 6, 8, 12, and 16 members (see 2 in 
fig. 3). All the Micrococci of these necklaces are distinctly held togther by a transparent 
connecting substance. The larger the necklaces grow, the more they become curved 
and convoluted (see 3 in fig. 3). 
It is worth noticing that in some instances the necklaces possess at one end or laterally 
at one point a comparatively large pear-shaped body, which consists of a greenish 
matrix, in which there is occasionally a highly refractive Micrococcus to be seen. 
The necklaces, which have grown to an immense length, and which have become con- 
voluted in a very complicated manner, are very liable to break up into a number of 
shorter chains ; in this case we have a convolution of necklaces from which several free 
ends stick out. 
( d ) If in such a convolution, consisting of a single chain or of several of them, the 
Micrococci become more and more closely packed together, and the connecting sub- 
stance of the individual chains becomes more and more coalescing, then we have a colony 
of Halliee or a zoogloea of Cohn. 
These changes of chains into colonies can be traced with great ease. 
( e ) There are many colonies which can be still recognized as being composed of 
necklaces closely packed together, and from which project shorter or longer filaments — 
in some places showing distinct divisions into rod-like joints, in others apparently smooth 
and homogeneous. I have tried to reproduce these features in fig. 3, 4, as accurately as 
possible. 
If the preparation is left a further twenty-four hours in the incubator and then 
examined, it is found that the isolated necklaces and colonies increase in number and 
size, whereas at the same time some of the Micrococci of the latter appear to become 
not only larger, but of a very great brightness and somewhat greenish. At the edges 
of the colonies, where the latter happen to project freely in small groups, we find them 
to possess a striking resemblance to those bodies represented in Plate 29. fig. 1, 7. 
At the same time we find a great number of perfectly transparent spheres, exactly 
similar to those described in the first preparation, and represented in fig. 1, 2, and fig. 2, ] , 
as far as size and aspect is concerned. 
They can be easily traced as being transformations of the spheres described on page 224 
and represented in fig. 3, 1 . The preparation having been kept in the incubator until 
March 28, i. e. during four days, was examined again, and it was found that the number 
of colonies was very great, that many of the Micrococci had become enlarged and of 
great brightness and of a greenish aspect. Besides, the filaments represented in fig. 3, 4, 
appear now to be very distinctly composed of rod-like joints, many of which have a more 
or less distinctly granular aspect. 
I have until now deliberately abstained from introducing any terms excepting “ Micro- 
cocci'' 1 and “ colony,” and I have tried to limit myself to a simple description of what 
