320 
REAGENTS. 
cent, of either of these salts, a drop of either hydro- 
chloric or sulphuric acid being used with a drop of the 
solution, according as the hydrochloride or sulphate 
has been used. 
The non-aniline stains give, as a rule, more reliable 
and constant results than the aniline stains. They in- 
clude the following: 
Beale's Carmine Solution, which is made as follows : 
Mix 06 gramme carmine with 375 grammes ammo- 
nia water (10 per cent.); heat on a water bath for 
several minutes; then add 60 grammes of glycerin, 60 
grammes of water and 15 grammes of alcohol, and 
filter. 
Grenacher’s Borax - Carmine Solution.— Dissolve 2 to 3 
grammes of carmine and 4 grammes of borax in 93 
c.c. of water and then add 100 c.c. of alcohol (70 per 
cent.); shake and filter. When this stain is employed 
the sections are freed from an excess by the use of 
alcoholic solutions of borax or oxalic acid. 
Hoyer’s Carmine Solution is an aqueous solution of an 
ammonium compound of carmine, to which 0 - 5 per 
cent, of ammonium carbonate has been added. An 
excess of the stain is removed from sections by the use 
of either alcohol alone or alcohol containing a small 
amount of acid. 
Acetic Carmine Solution is made by adding acetic acid 
to Hoyer’s carmine solution until the solution be- 
comes of a brick-red color. For washing the sections 
a solution of glycerin containing 0 - 5 per cent, of hydro- 
chloric acid or 1 per cent, of formic acid is employed. 
Hoyer’s Piero Carmine Solution is made by dissolving 
carmine in a concentrated solution of neutral ammo- 
nium picrate. A solution of carmine and picric acid 
is known as “ Piero-Carmine Solution.” 
