STAINING AGENTS. 
321 
P. Mayer's Carmine Solution. — Four grammes of car- 
mine are mixed with 15 c.e. of water and 30 c.c. of 
hydrochloric acid, and the mixture heated on a w T ater- 
bath; to this 95 c.c. of alcohol are added and ihe solu- 
tion boiled, neutralized with ammonia water (10 per 
cent.), and filtered when cool. 
Chlor-zinc-iodide Solution, or Schulze’s Cellulose Re- 
agent, consists of anhydrous zinc chloride, 25 grammes; 
potassium iodide, 8 grammes; and water 8’5 grammes, 
to which as much iodine is added as it will dissolve. 
Bohmer’s Hsematoxylin Solution is prepared by mixing 
the two following solutions and filtering after standing 
for several days : (a) one part of a 3 5 per cent, alcoholic 
(95 per cent.) solution of heematoxylin and (6) three 
parts of a 0 - 4 per cent, aqueous solution of potassium 
alum. 
Delafield’s Haematoxylin Solution, which is also incor- 
rectly called “ Grenacher’s Haematoxylin Solution,” is 
made by mixing the follow r ing solutions : (a) Hsema- 
toxylin 4 grammes, alcohol 25 c.c. and ( b ) 400 c.c. of a 
saturated aqueous solution of ammonia alum ; this solu- 
tion is exposed to the light for three or four days, 
filtered, and then 100 c.c. each of glycerin and methyl 
alcohol are added, the solution allowed to stand for 
several days and finally filtered. An excess of the 
stain i3 removed from the sections by subsequent 
washing either with a 2 per cent, alum solution or an 
acidified alcoholic solution. 
Iodine and Potassium-Iodide Solution consists of iodine, 
13 grammes; potassium iodide, 20 grammes; water, 
100 c.c. 
Iodine Water is prepared by adding as much iodine 
to distilled and sterilized water as it will dissolve (about 
1 : 5 , 000 ). 
