324 Wager. — On the Presence of 
one side of the nucleus. These different structures behave 
towards the double stain as follows : — The protoplasm is pale 
red with a bluish tinge; nucleolus deep blue-red; network 
also bluish red or blue. 
Just outside the nucleus and generally in close contact 
with it are one or two bodies of somewhat large size, generally 
about the same size as the nucleolus when only one is present, 
a little smaller when two exist. They are coloured light blue 
and are easily overlooked, especially in badly stained or only 
slightly stained specimens. These bodies were always seen 
at this stage (see Figs. 8 and 9), in all those cases where the 
nuclei could be distinctly observed. Each appears to consist 
of a homogeneous mass of substance, spherical in outline. 
When two of these bodies occur, which is a preliminary stage 
to that when only one is present, they are not always in the 
same position. Sometimes both of them may be found on 
the upper side of the nucleus, or laterally, or they may occur 
one at each end of the nucleus, and generally they are in close 
contact with it ; only occasionally are they removed a slight 
distance away. So far as the structure of these bodies is 
concerned, it differs considerably from that described by 
Guignard and others as existing in the centrospheres of plant- 
cells. There is no indication of a medullary corpuscle or 
centrosome. The bodies simply present the appearance of 
the so-called ‘ Nebenkern ’ of many observers, and answer more 
nearly to the description given by Hermann 1 of the archo- 
plasmic bodies in the spermatocytes of Salamander and 
Proteus, or in the cells of the embryonic genital ridge of 
the larval Salamander as described by Moore 2 . The latter 
observer points out that in these cells the archoplasm is 
a large pale grey mass, nearly as large as the nucleus itself ; 
but when most conspicuous it is relatively small, and presents, 
in this condition, even with the highest powers, only doubtful 
suggestions of medullary zone or central body. We have 
1 Archiv fur mikroskopische Anat. XXXVII. 
2 Quart. Jour. Microsc. Sci. 1893. 
