Centro spheres in Fungi . 327 
process of separation of the threads, until the groups of 
chromatic elements begin to fuse together at the poles (Fig. 20) ; 
after that they become invisible. I could not determine 
whether they disappear altogether or not (Fig. 21). 
The two daughter-nuclei are gradually formed as described 
in my previous paper, but I could not at any stage detect 
anything of the nature of an archoplasmic body in contact 
with them. This might be due to the difficulties in the way 
of preparation or staining. At a later stage, when the spindles 
of the daughter-nuclei appear, two centrosomes can be made 
out at the poles of the spindle, but these are not quite so clear 
as in the original parent-nucleus. The four daughter-nuclei 
produced, gradually expand until they become similar to the 
parent-nucleus. The various stages are figured in Figs. 23-29, 
but at no stage of their development could anything of the 
nature of an archoplasmic body (as distinguished from the 
centrosomes) be made out. This, however, is not equivalent 
to saying that archoplasmic bodies or centrospheres are not 
present. It may be that owing to imperfections in the method 
of preparation they were not rendered visible, or some change 
may have taken place of which we are not cognizant, owing to 
our scanty knowledge of archoplasmic structures and their 
nature. We cannot therefore at present state what is the 
ultimate fate of the archoplasmic body in A. galericulatus , 
and we are in much the same difficulty as regards its origin. 
In fact the whole question of the origin of the centrospheres, 
whether they are derived from the cytoplasm or from the 
nucleus, cannot yet be satisfactorily answered. Some observers 
incline to one view, some to the other. It certainly appears 
as if, in the majority of cases, the centrospheres were always 
outside the nucleus, and Guignard’s observations favour this 
view for plant-cells 1 ) but in animal cells there are cases where 
the centrosphere appears to be distinctly nuclear in origin. 
August Brauer 2 describes the centrospheres in the spermacyte 
1 Loc. cit. 
2 Archiv f. Mik. Anat. 1893, pp. 285-7 > Bio- Cent. 1893, p. 197. 
Z 
