24 Ward. — Recent Researches on the Parasitism of Fungi. 
All gave negative results , although in the fourteenth case further infections were 
made with teleutospores on Sept. 22 and 24. On Mar. 29, 1893 = plants still growing, 
but died soon after. No rust. 
i. e. at least 126 sown and 126 failures. 
(c) Spring Cereals in cases in summer, 1893. Used 7 cases. All began 
May 15. 
Case. 
Soil. 
Seed. 
I 
Not sterilized 
5 Barley, showing Pucc. glumarum. 
2 
Sterilized 3 h. 
)) 5 ) 
3 
Not sterilized 
5 Barley, free of Fungus. 
4 
Sterilized 3 h. 
5 
Not sterilized 
5 Oats, free of Fungus, but added Puccinia. 
6 
Sterilized 3 h. 
) 5 »> >> 
7 
>> 
5 Oats, free of Fungus, and nothing added. 
Results totally negative to Aug. 9. 
i. e. 35 sown and 35 failed. 
(d) Autumn Wheat in long tubes in spring of 1893. 
Used 10 seedlings, each in a tube, but although they got Mildew ( Erysiphe ) 
they had no rust. N.B. Eriksson points out that there was very little rust anywhere, 
as it was a poor year for the Fungus ! 
i. e. 10 seedlings and 10 failures. 
(e) Autumn Wheat in ventilated boxes, autumn, 1893. Ten cases used. 
Case. 
Sown. 
Soil. 
Seed. 
I 
Aug. 29 
Not sterilized 
9 seeds showing Puccinia . 
2 
V 
Sterilized 3 h. 
a }) 
3 
>} 
Not sterilized 
9 seeds, free of Fungus. 
4 
j) 
Sterilized 3 h. 
» }i 
5 
a 
55 
Whole spike rusted. 
6 
>} 
)> 
9 seeds showing Puccinia and sterilized 3 h. at 
48° dry. 
7 
)) 
>> 
9 grains. These were inoculated on Sept. 8 on 
roots or leaves with uredospores. 
! 8 
Sept. 4 
)} 
9 grains, free of Fungus, also inoculated on 
Sept, io-ii with teleutospores. 
9 
>> 
)} 
9 grains, Fungus free. 
10 
}) 
)> 
Here, although the cultures in the cases were in every respect excellent, yet 
none showed rust. In no case were positive results obtained. The only remarkable 
point here is that of case 7, where the after-inoculation with uredospores also failed, 
i. e. at least 90 sowings and 90 failed. 
