Ward. — Recent Researches on the Parasitism of Fungi. 23 
Total Experiments. 
Year. 
No. of Plants. 
Successes. 
Failures. 
(a) 
1892 
15 
0 
15 
(b) 
1892-3 
126 at least 
0 
126 
( c ) 
1893 
35 
0 
35 
w 
1893 1 {tubes) 
10 
0 
10 
( e ) 
1893 
90 at least 
0 
90 
(*) 
1894-9 2 {tubes) 
32 at least 
7 
25 
(g) 
1894 3 
14 
2 
12 
(h) 
1894 
20 
0 
20 
(i) 
1895 
20 
1 
19 
(j) 
1897 4 
14 
9 
5 
(k) 
1898 
i 5 
0 
15 
Total . . 
39 1 
19 
37 2 
(a) Oats in cases, summer 1892. To see if they can be cultivated in cases, and what 
importance may be attached to the presence of spores in soil. Three cases. One — 
5 seeds Oats covered with T. repens + Black Rust. Two = 5 seeds in sterile soil. Sowed 
June 5. No trace of rust to August. Eriksson says all grew too abnormal to develop 
the mycoplasm germ out. Yet inoculations on Aug. 23 and 24 gave + results in 
10-20 days ! 
i. e. 15 seeds sown and all proved failures. 
(b) Autumn Wheat in cases in autumn 1892 and through winter to 1893. 
Michigan Bronze and Yellow Rust, to which it is susceptible. 14 cases. Questions : 
(1) Can seed or seedling be contaminated by Uredo glumaruml (2) Or by Pucc. 
glumarum ? (3) Can disease proceed from diseased seed? (4) Can disinfection 
(heat dry, or Jensen’s hot water) of seed destroy the Fungus ? 
Case. 
Began. 
Soil. 
Seed. 
I 
Sept. 12 
Not sterilized 
9 seeds with Pucc. glumarum. 
2 
JJ 
Sterilized 5 h. 
jj jj 
3 
JJ 
Not sterilized 
„ no teleutospores. 
4 
JJ 
Sterilized 5 h. 
jj jj 
5 
JJ 
jj 
„ no teleutospores, and disinfected by 
Jensen. 
6 
Sept. 13 
jj 
„ with teleutospores and disinfection dry, 
4 h. at 40-43°C. 
7 
jj 
jj 
Whole spike with Pucc. glumarum. 
8 
>) 
Not sterilized 
9 seeds quite free of rust. 
9 
Sept. 14 
Sterilized 5 h. 
jj jj 
10 
Sept. 15 
jj 
„ „ but Ur edo glumarum added. 
11 
jj 
jj 
„ „ but added Puccinia. 
12 
Sept. 16 
jj 
9 seedlings devoid of Fungus, but added uredo- 
spores. 
13 
>j 
jj 
9 seedlings and added teleutospores. 
14 
Sept. 17 
jj 
9 seedlings and no addition. 
1 No rust but abundance of ErysipheX 2 In midst of badly infected rusted Wheat. 
3 Cases appear to be openly ventilated, 4 Aphides in one of the successful cases. 
