136 Salmon— Further Cultural Experiments with 
patches, with large lobed haustoria, were observed, on three of the leaves, 
proceeding from conidia sown on the bruised cells themselves. On the 
fifteenth day one leaf bore several mycelial patches with a few weak 
scattered conidiophores ; on the two other leaves only a few small barren 
mycelial patches had been formed. No infection occurred on the other 
leaves. It was evident that the injury inflicted was not sufficient, in 
most cases, to render the leaf susceptible. 
In the next experiment (No.«i) three barley leaves were bruised more 
severely. Inoculation was made on the bruised cells on the upper surface 
of two leaves, and on the lower surface, on cells opposite the bruise, in the 
third case. On the fourth day numerous vigorous little patches of mycelial 
hyphae were visible on the upper surface of the two leaves, on the bruised 
cells themselves. On the sixth day several of these patches bore a few 
conidiophores. On the eighth day one leaf bore numerous vigorous little 
tufts of conidiophores on the bruised cells themselves ; a few conidiophores 
had been formed, also, on the bruise on the second leaf; on the third leaf, 
on the lower surface, no infection had occurred. On the thirteenth day the 
three leaves were translucent throughout ; the two leaves inoculated on the 
upper surface bore vigorous little conidiophore-bearing patches of mycelium 
on the bruised cells and on those immediately surrounding them ; no 
infection resulted from the conidia sown at distances of 2 mm. and 
3 mm. from the bruise. The leaf inoculated on the lower surface, 
opposite the bruise, bore one little barren patch only, of a few mycelial 
hyphae. 
In the third experiment (No. a 13) three barley leaves were bruised on 
the upper surface, and a space 0-5 cm. wide was marked off at either side 
at a distance of 0-5 cm. from the edge of the bruise. Inoculation was made 
both on the bruised part and on the spaces marked off on either side. On 
the sixth day infection had resulted on the bruised place on all the leaves, 
both on the bruised cells themselves and on those immediately surround- 
ing them. On the eighth day small vigorous mycelial patches, mostly 
bearing a few conidiophores, occurred on all the leaves. No infection 
occurred at the marked places 0-5-1 cm. distant from the bruise. 
In a further experiment (No. a 8) one barley leaf was severely bruised 
and inoculated over the bruise. By the tenth day a few conidiophores and 
several patches of mycelium were visible on the cells immediately surround- 
ing the bruise. 
In one experiment (No. 13) a leaf attached to a seedling plant of 
barley, about three weeks old, was bruised. The leaf was inoculated at 
the bruised place, and laid on damp blotting-paper at the bottom of 
a Petri dish, the lid being placed over it. The roots of the seedling plant 
were kept in water. On the sixth day the bruised place was covered with 
very vigorous but barren mycelial patches. By the tenth day the mycelial 
