140 Salmon. — Further Cultural Experiments with 
bearing a few conidiophores were visible. In this experiment the leaves 
used were rather old. The conidia produced on the barley leaves were sown 
on four leaves of barley and of wheat, and proved able to infect only the 
wheat. 
In experiment No. a 77, three barley leaves were again used. On the 
ninth day two of the leaves appeared fully infected, and bore hundreds of 
conidiophores in little clusters scattered over the inoculated surface ; on the 
third leaf only a few scattered almost solitary conidiophores appeared. In 
this experiment again oldish leaves were used. Conidia taken from the two 
barley leaves and sown on two uninjured leaves of barley and of wheat 
proved able to infect only the wheat. 
In experiment No. a 78, three barley leaves were again used, and all 
became apparently fully infected, bearing on the eighth day numerous little 
clusters of conidiophores and patches of mycelium over the sown area. 
In experiment No. a 82, three barley leaves were used, and two of these 
on the tenth day presented the appearance of being fully infected. In this 
experiment three control leaves (rather old) of barley were inoculated. On 
two of these a few isolated single conidiophores appeared. 
In experiment No. a 09, twelve barley leaves were rubbed lightly in 
distilled water to remove the adherent film of air ; six were then immersed 
in the alcohol for 4 hours. After being dried, these six leaves were 
inoculated, together with the other six leaves, and all were placed side by 
side at the bottom of two Petri dishes. On the ninth day, in one Petri 
dish, two of the leaves which had been treated with alcohol bore a number 
of conidiophores, and one control leaf bore four isolated conidiophores. In 
the second Petri dish, all the treated leaves bore the appearance of being 
fully infected, the inoculated area of each leaf bearing many hundreds of 
conidiophores. No trace of infection occurred on the three control leaves. 
On the fourteenth day one of the treated leaves in the second Petri dish 
was covered over its surface for a distance of 3 cm. with many hundreds of 
conidiophores. No trace of any infection appeared on the three control 
leaves. Conidia from the treated barley leaves were sown on two uninjured 
leaves of barley and of wheat, and infected only the latter. 
In experiment No. a 01 1, three treated leaves and three controls were 
inoculated. On the eighth day the three treated leaves bore scattered 
conidiophores over the inoculated surface. By the thirteenth day the 
three treated leaves bore numerous conidiophores, sometimes in little 
groups, over the inoculated place ; no trace of any infection appeared on 
the controls. 
Immersion of barley leaves for 2 hours in a 10 °/ o mixture of alcohol was 
made in one experiment (No. a 66 ), in which three leaves were used. On 
the ninth day a few scattered, isolated conidiophores were visible on all the 
leaves. On the fifteenth day two of the leaves bore little clustered groups 
