142 Salmon. — Fur the}' Cultural Experiments with 
lated. In experiment No. a 86, the three barley leaves thus treated all 
became infected, at the marked places, by the seventh day. On one leaf 
numerous mycelial patches and hundreds of conidiophores, often in little 
clusters, were produced ; on the two other leaves a few scattered, sub- 
solitary conidiophores appeared among the sown conidia. In experiment 
No. a 07, four barley leaves were gently rubbed in water to remove the 
adherent film of air. One leaf was then put into a vessel of cold water, and 
the water heated slowly to 50° C. ; the leaf was then taken out, dried, and 
inoculated. The three other leaves were at the same time removed from 
the cold water, dried, and inoculated. On the ninth day the heated leaf 
bore, over the inoculated surface, thousands of scattered conidiophores ; none 
of the controls was infected. In experiment No. #010, six barley leaves 
were rubbed in water to remove the air-film, and three were then slowly 
heated in water to 50° C. ; all the six leaves were then dried and inoculated. 
On the ninth day the three heated leaves all bore very numerous conidio- 
phores, and presented the appearance of being fully infected. On one 
control leaf three isolated conidiophores appeared. In experiment No. a 033, 
three barley leaves were put straight into cold water, and heated slowly to 
50° C. The leaves were then taken out, dried, and inoculated. The 
heating took 1 hour 25 minutes. During this time three control barley 
leaves stood immersed in a vessel of cold water ; these also were dried and 
inoculated at the same time. On the eighth day numerous scattered 
conidiophores, and patches of mycelium, were visible on the three heated 
leaves ; no signs of infection were visible on the controls at this date. On 
the thirteenth day the controls had turned yellowish, and were completely 
translucent ; on two leaves a conidium was visible, which had germinated 
and produced a few short spreading hyphae, and in one case two conidio- 
phores had been produced from the hyphae. 
In experiment No. a 0382, six barley leaves were cut off from 
strong flowering plants, 3 feet high, and 5 months old, growing in the open. 
Three leaves were immersed in cold water, and the water heated slowly to 
50° C. All six leaves were cut into pieces about 8 cm. long, inoculated, and 
placed in a Petri dish. On the seventh day two of the treated leaves bore 
many scattered little patches of mycelium, several of which bore a few 
clustered conidiophores. No infection occurred on the controls. 
In one experiment (No. a 036) ascospores were used. Six barley leaves 
and two wheat leaves were used ; three of the barley leaves were put 
straight into cold water, and the water heated gradually to 50° C. The 
leaves were then taken out and dried, and together with the three control 
barley leaves and the wheat leaves, were exposed for 48 hours to ripe, 
bursting perithecia, ejecting ascospores. On the eighth day the two wheat 
leaves bore numerous powdery Oidium-patch.es scattered over the inoculated 
surface; no signs of any infection appeared as yet on the barley leaves. 
