144 Salmon . — Further Cultural Experiments with 
numerous flecks of mycelium with young conidiophores. On the seventh 
day all the leaves bore the appearance of being fully infected ; in one case 
the conidia, produced in little powdery masses on the clustered conidio- 
phores, were so numerous that they could be removed in little heaps with 
the blade of a scalpel. On the twelfth day this one leaf still bore quite 
powdery little clusters of conidiophores. Conidia were twice taken from 
the barley leaves and sown on two uninjured leaves of barley and of wheat ; 
they proved able to infect only the wheat. 
In experiment No. a 049, three barley leaves, after being immersed in 
a 10 °/ o mixture of alcohol for 4 hours, were put into cold water and heated 
slowly to 5o°C. All the leaves were killed. 
Oat leaves were used in three experiments. In the first (No. a 032) 
six oat leaves were slowly heated in water up to 50° C. On the tenth day 
two of the leaves bore a few small radiating mycelial patches, one of 
which, on each leaf, bore two conidiophores ; small barren mycelial patches 
occurred on two of the other leaves. This experiment was repeated 
(No. #0367). On the seventh day four of the leaves bore numerous, 
little, spreading mycelial flecks, all barren, except two, on one leaf, which 
bore five and three conidiophores. In experiment No. a 062, six oat leaves 
were heated slowly to 55 0 C. ; the leaves were killed by this treatment. 
Leaves of Agropyron repens were used in one experiment (No. a 089). 
Three oldish leaves were slowly heated in water to 5°°C. They were then 
cut transversely into half, inoculated, and placed at the bottom of a Petri 
dish. On the seventh day most of the leaves were flaccid and dying or 
dead. One leaf, however, was not killed, and was still rigid, and bore 
numerous mycelial patches and clusters of conidiophores, presenting, in 
fact, the appearance of being nearly fully infected \ On two other nearly 
dead leaves patches of barren mycelium were visible. 
In experiment No. a 097, four pieces of the underground rhizome of 
Elymus arenarius were heated in water to 5 L ° C. A small piece of tissue 
was then cut away from the side of each piece, and inoculation made on 
the cut surface and also over the uninjured surface of the rhizome. On the 
third day minute patches of mycelial hyphae were visible on two of the 
pieces of rhizome, and on the fifth day a few conidiophores. On the 
seventh day, many hundreds of conidiophores were visible on one piece 
of the rhizome, close by the sides of the cut, but not on the surface 
of it. 
1 Conidia from wheat are not able to infect normal leaves of A. repens (see 1 Beihefte z. Botan. 
Centralbl.,’ xiv, 308, Tab. 10). 
