Pollen Mother-cells of Lilhim coincide use. 217 
Such appearances as those of Figs. 79 and 88 would lead to the 
conclusion that the newly-formed segments of the daughter chromosomes 
remain attached at the angle of the V — in other words, that the second 
longitudinal split is not completed, at least in this mitosis. This is the 
regular appearance if the chromosomes are viewed, as is the case in these 
figures, from the exterior of the spindle. But a very different impression 
is gained (Fig. 80) by observing that side of the chromosomes turned 
inward. It will be remembered that the slight hook which is commonly 
formed upon the polar ends is usually turned inward. The hooked shape 
of the daughter chromosomes is shown in Fig. 80, and it is plain that each 
is split throughout its whole length, the polar end of each granddaughter 
chromosome being turned inward, its equatorial end, as we have seen, 
turned outward. It would seem that this inward hooking of the polar 
ends, with often more or less overlapping of the hooked ends of the grand- 
daughter chromosomes, obscures the view of the splitting from the outer 
side, and gives the appearance of a continuity between the two arms of the 
V at the angle. At the angle of each V in Fig. 79 there is a slight 
indentation, which is doubtless an indication of the completed splitting. 
It is not always the case that an interior view shows the completed splitting 
so plainly as does Fig. 80, but such figures are so common that there is no 
doubt that the complete separation of the granddaughter chromosomes 
in the anaphases of this division is the general rule. It will be seen that 
the splitting of the daughter chromosomes shown in Fig. 80 has been 
accompanied by the separation of the spindle fibres into distinct clusters, 
one cluster attached to each granddaughter chromosome. 
The appearances just described are characteristic for the large majority 
of the chromosomes ; but in cases of the attachment of the spindle fibres 
otherwise than at the ends of the daughter chromosomes (Figs. 65-67) the 
resulting figures are somewhat different. Figs. 81 and 83 represent later 
stages in the separation of such chromosomes ; and it will be seen that, as 
the daughter chromosomes retreat toward the poles, a bend occurs at the 
point of attachment ; that is, the more common hooked form is exaggerated, 
and each daughter chromosome consists of two arms whose relative length 
depends upon the point of attachment of the fibres. The result of the 
splitting of such daughter chromosomes is shown in Figs. 82 and 86 ; the 
derivation of Fig. 82 from such a form as is shown in Fig. 81 is plain ; and, 
on the other hand, Fig. 82 differs from the commoner type (Fig. 80) only 
in the greater length of the hook at the polar end of each granddaughter 
chromosome. If the fibres are attached near the middle, the two arms of 
each daughter chromosome, and, after the second longitudinal split, those 
of each granddaughter chromosome, will be of about equal length ; each 
daughter chromosome will then (Fig. 85) consist of two V’s with their 
angles in contact and turned toward the pole, and with their arms diverging. 
