34i 
Campbell. — Studies on the Araceae , III ‘ 
was a single apical aggregation of cytoplasm with a nucleus, the whole 
probably being the egg. Nothing which could be Interpreted as synergidae 
could be seen. In the upper part of the undivided cavity of the sac was 
a large nucleus in process of division, and nearer the base a second free 
nucleus (e. n.) in a resting condition, was seen. There was no sign of nuclear 
fusion, but it is possible of course that the division of the upper free nucleus 
may have been preceded by a fusion of two or more nuclei. A little 
at one side of the base of the sac was a nearly hemispherical cell mass (an.) 
containing, as nearly as could be determined, twelve cells. These perhaps 
represent the antipodal cells. The twelve rmclei belonging to this cell 
mass, together with the three in the upper part of the sac, make a total 
of at least fifteen nuclei for the unfertilized sac, even if there had been 
no nuclear fusions. The absence of the synergidae, and the very slight 
polarity in the arrangement of the nuclei, suggest Peperomia in this 
case also. 
The sac shown in Fig. 47 exhibited at the apex a large cell containing 
what seemed to be two large nuclei in process of fusion. The rest of 
the sac was divided obliquely by a delicate membrane, the upper division 
containing a single nucleus, the lower three nuclei, two of which were 
fusing. No antipodal cells could be seen. 
The sac shown in Fig. 46 approached the normal angiospermous type. 
At the apex were three cells resembling closely a normal egg-apparatus. 
At the chalazal end of the sac were two large antipodal cells with rather 
small, but strongly staining nuclei. In the middle of the sac were three 
large free nuclei. The lowest of the three was smaller than the others, 
and may have represented a third antipodal. The two larger free nuclei 
were widely separated and the upper one was evidently preparing to divide. 
If these two nuclei represent the polar nuclei of the ordinary embryo-sac, 
it is pretty clear that there is no fusion of these preliminary to the first 
division of the endosperm. Although in this case there were the typical 
eight nuclei present, the indicated division of one of the polar (?) nuclei 
showed that the number would be increased before fertilization took place. 
Another case met with was difficult to interpret. It is not impossible 
that there was here an aggregation of several sacs, and not a single one. 
At the apex were two cells, apparently with cellulose membranes, one 
with dense contents and somewhat contracted nucleus, the latter perhaps 
owing its appearance to the action of reagents ; the other with much less 
granular cytoplasm and perfectly normal nucleus. The space below 
was incompletely divided, and at the base was a group of three cells, 
one larger and with more conspicuous nucleus, the whole looking like 
a normal egg-apparatus. Near this egg-apparatus (?) was a second group 
of five cells with large nuclei, and probably the antipodal cells. Nothing 
comparable to polar nuclei was visible. It Is possible the two basal groups 
