184 Clifford. — Notes on some Physiological 
in each case was sudden and decided, and a rapid creeping 
movement away from the heat took place. The plasmodium 
must in all this work be kept well supplied with moisture, 
else it either dies or assumes the sclerotium form. These 
facts may be taken as another evidence of the extreme sensi- 
tiveness of the organism and its power to protect itself against 
unfavourable conditions. 
Raising the temperature by means of an additional flame 
applied directly under the box used, to about 48° C., was 
followed by a cessation of all apparent movement ; and upon 
raising it still higher, it was found that the plasmodium dies 
from a very short exposure to a temperature of 52°-53° C. 
Woodcut 5. 
Results obtained from other Temperature-changes. 
After trying various contrivances for microscopic exami- 
nation of the behaviour of plasmodium, the following was 
found most satisfactory : a glass slide, 3 in. x 2 in., and a 
cover-glass, 2 in. x 2 in., were separated by a double layer 
of blotting-paper one-fourth of an inch wide, as shown in 
Woodcut 5. In this way a small cage is formed sufficient to 
contain the necessary moisture, and as the plasmodium is not 
in the least geotropic, the thin glass with the plasmodium 
