NOTES. 
FUNGI FOR CLASS-DEMONSTRATION.— The following 
may possibly be useful to others who, like myself, are concerned in 
the preparation of class-material. 
Bunt . — To obtain material for Tilletia , I break up three or more 
bunted grains of Wheat in water in a small evaporating dish ; weigh 
or count out the seed-wheat required and add it to the water (the 
amount of which depends on the quantity of seed used) ; stir 
thoroughly and carefully, and allow to dry, stirring frequently as 
drying proceeds, so as to get the spores well distributed instead of 
allowing them to settle where the film of water over the grain is 
in contact with the dish or with other grains. I usually do this in the 
afternoon, so that the grain is dry enough for sowing on the following 
morning. 
The effect of a copper-sulphate dressing is easily shown by taking 
part of the Wheat thus contaminated and dressing it with copper- 
sulphate in the proportion used by farmers, and sowing it when dry 
in a row next my control row. In the past three years I have found 
no instance of the failure of the copper-sulphate dressing to prevent 
Bunt in Wheat. I keep my stock, for class-work on the spore- 
germination, in covered glass jars. 
Smut . — The method adopted for Bunt was a failure as regards Smut. 
Nor did I obtain very great success by soaking and opening the seed- 
grain and actually placing the spores on the embryo. The plan I now 
follow is to soak Barley in water for about twenty-four hours : I then 
remove the chaff and stir up the naked kernels in a small amount of 
water, in which I have broken up several grains of smutted Barley ; 
allow to dry, and sow when dry. My Barley tillers well, so to obtain 
[Annals of Botany, Vol. XI. No. XLII. June, 1897.] 
