62 
Sharpies and Lambourne. — Observations in 
falling over on their own. However, the position is very different on 8.3.21, 
when these trees appeared to have recovered to some extent. The manner 
of recovery will be discussed below. 
The trees in Group C behaved in much the same manner as those in 
Group B y though the initial severity of attack was not so noticeable. 
During the experimental period five out of six trees were attacked, but on 
8.3.21 the five trees still remaining were quite healthy. 
Group D, inoculated with a mixture of B and C, shows a different 
course of events, the attack being slowed up considerably. On 8.3.21, 
of the five trees remaining, all showed evidence of attack. This result is 
probably connected with the initial hold-up; it is to be expected that all 
these trees will recover. 
The twelve controls established for the bacterial infections show 
definitely that without inoculating material the infections are neither so 
rapid nor severe. It is to be expected that the gouge going through the 
leaf-bases will, in some cases, carry material into the centre which, on 
reaching the nutritive bud-tissue, will ultimately develop and possibly cause 
a Bud-rot. In these twelve controls only two showed definitely on 
8.10.2c, with one mild attack. On 9. 11.20 another tree was badly attacked, 
and another very mildly attacked, and one of the previous- badly attacked 
trees was recovering. On 8.3.21 only one showed a bad attack with three 
mild attacks. 
The results brought forward undoubtedly proved the point we set out 
to establish, i. e. ‘ that no conclusions based on “ stab inoculations” can be 
considered satisfactory, when the inoculated tissues are rich in easily 
converted food materials \ 
This point is of the utmost importance in a consideration of Bud- 
rot of palms, for many investigators working on this problem have based 
their conclusions largely upon results obtained by ‘ stab inoculations ’. In 
order to establish the point more firmly, further inoculations were made 
with three different organisms commonly appearing on rotting bud-tissue 
in the laboratory. These inoculations were performed some two months 
after the first set, so that any change of conditions might be expected to 
influence these later results. The groups follow on in order : 
Group E inoculated with Thielavia sp. 
„ F „ ,, Mucor sp. 
„ G „ ,, Bacillus flavo-coriaceus. 
The results of the later experiment are entirely concordant with the 
previous one. The Group E inoculations went off quickly, four trees being 
badly attacked, but on 8.3.21 all except one were recovering — one 
remained healthy throughout. The sequence of events parallels the 
Group B inoculations. The Group E inoculations started very slowly 
