64 Sharpies and Lambourne. — Observations in 
and on 9.1 1.20 the trees appeared quite healthy. The second inspection 
on 8.3.21 revealed four very badly and one mildly attacked and one 
healthy. The further course of this group will be watched. The sequence 
is similar to that observed in the mixed Group D inoculations. 
The Group G inoculations showed three badly infected cases on 
9.1 1.20, but on 8.3.21 only one remained in a badly diseased condition, 
while three others which had suffered were well on the road to recovery ; 
only one was healthy throughout, and one was cut out for examination. 
The controls were again influenced by beetle attack, noted previously 
in the Group A controls. On 9.1 1.20 there were only three mild 
attacks and three attacked by beetles ; these latter were otherwise healthy. 
On 8.3.21 there was only one badly diseased tree, three slightly 
attacked were almost recovered, and one still showed a mild attack with no 
obvious attempt at recovery. 
Interpretation of Results. 
The essential point in interpreting the foregoing results is the recognition 
of what is to be considered a successful inoculation. The writers have 
stated their guiding line (supra), drawing upon the experience of previous 
investigators. Johnstone ( 4 ) has further remarks upon the difficulty of 
inoculating the heart of a Coco-nut tree. He says, ‘ Inoculations made 
below the heart fail to produce the rot, since these tissues naturally soon 
harden as a part of the mature trees. If, on the other hand, the inoculation 
be made above the heart, amid the growing leaves, their extremely rapid 
elongation takes the inoculation point out of the surrounding soft tissues. 
The inoculated tissues then become green and membranous, and thus resist 
the advance of the rot.’ 
Johnstone ( 4 ), when considering inoculation experiments, rightly stresses 
the necessity for the actual rot of the bud-tissue. As far as our acquaintance 
with the literature goes other investigators have been satisfied to produce 
a rotting and falling over of the central heart leaves — in such case our 
inoculations marked 0 could not be misinterpreted. 
The plates show some of our typical results. Plate II shows a tree 
from the Group G inoculations, the central leaves leaning and about to fall. 
Plate III shows the central leaves fallen out of a tree in Group C. Plate IV 
shows one of the control trees which became infected with the centre falling 
out. Plate V shows a similar effect after a Phytophthora faberi inoculation 
(Group A), and Plate VI shows another tree inoculated with the same 
fungus. These photographs can leave no possible doubt as to our success 
in obtaining what are usually considered definite signs of Bud-rot. 
As indicated in the tables, trees from the various groups were taken out 
at intervals to note the progress of the inoculations. On 8.10.20 two trees 
