6 ; 
Malaya on Bud-rot of Coco-nuts . 
along the lines of the present writers, by trying to induce rotting of the 
bud-tissues on mature trees by artificial stab inoculations. His conclusions 
are based on results in which the time period allowed between inoculation 
and examination was generally from eight to sixteen days. He also records 
two other inoculations: one performed on June 22, with a bacterial culture, 
showed a Bud-rot on October 21 ; the second, similarly treated on July 22, 
showed no evidence of Bud-rot on August 6 . As stated, too much 
importance cannot be attached to results and conclusions when the period 
elapsing between inoculation and examination is so short, for it is obvious 
from the inoculations recorded above that, Iven if organisms are introduced 
directly into the bud-tissues, their behaviour may be very different. The 
Group D and Group F inoculations were significant in this respect, for the 
period elapsing between inoculation and the first signs of diseased tissues 
was well over two months, whereas in all the other groups the first signs 
were almost immediate. 
Shaw and Sundaraman ( 8 ), working with Butler’s Pythium (Phyto- 
phthora) palmivorum, when considering Bud-rot of Coco-nuts, obtained results 
similar to those of Butler in his work on Palmyra Palm Bud-rot. They 
obtained depressed spots without wounding on the tender central leaves, 
indicating a parasitic penetration by the fungus. They also obtained 
collapse of the central shoot in a seedling Coco-nut when inoculated and kept 
in a damp chamber. 
Their inoculation experiments were wholly upon seedlings, no mature 
palms being utilized. The only admissible conclusion here as in the 
previous work of Butler, is that inoculations with Phytophthora palmivora 
(Butler) result in a decided parasitic penetration of the central leaves, 
without any necessary connexion with rotting of the ‘ Bud ’. 
Reinking’s work has already been considered. In view of the fact that 
his inoculations were practically all stab inoculation on seedling Coco-nuts, 
the evidence brought forward for Phytophthora faberi as the cause of 
Bud-rot in the Philippines cannot be considered satisfactory, and no 
definite conclusion as to the cause of Bud-rot can be arrived at. As 
regards our Phytophthora faberi (Maubl.) inoculations, they were no better 
or worse than inoculations made with the other recorded organisms. Most 
are recovering, and, although the only genuine Bud-rot occurred in this 
group, a successful inoculation on one tree is not sufficient to base 
important conclusions upon, when the remainder behave as other groups 
inoculated by widely different organisms. 
Ashby’s work ( 1 ) is of interest as confirming Butler’s ( 2 ) and Shaw r 
and Sundaraman’s work ( 8 ). He records similar features, and from his 
observations fairly concludes that inoculated trees showing rows of typical 
Phytophthora palmivora spots on central leaves do not necessarily die at 
the heart. 
