442 
Campbell . — The Gametophyte and Embryo of 
carries a remarkable Pteridophytic flora. It is also remarkable in that 
many of the flowering plants of the northern woods occur here. The orchid 
flora is particularly rich and interesting.’ 
The gametophyte of B. simplex is much like that of the other species 
that have been described , 1 being on the whole most like that of B. lunaria , 
as might be expected. Bruchmann de- 
scribes the latter as a small subterranean 
tuber-like body, 1-2 millimetres in length. 
In all these respects B. simplex agrees 
closely with B. lunaria , except that some of 
the specimens examined by the writer were 
slightly larger than the dimensions given for 
B. lunaria , reaching a maximum length of 
about three millimetres. Bruchmann shows 
figures of several cases where there ap- 
parently had been a dichotomy of the apex 
of the gametophyte, which had two short 
divergent branches, so that the gametophyte 
was heart-shaped. This condition was not 
seen in B. simplex , but it is quite possible 
that it may occur. 
In both B. simplex and B. lunaria , which 
belong to the section Eubotrychium , the 
gametophyte is much smaller than in either 
B. obliquum or B. virginianum , representing 
respectively the sections Sceptridium and 
Osmundopteris? 
Fig. 1 of Plate XVI shows one of the 
youngest specimens found in the material 
examined. Seen from above it is nearly oval 
in outline, slightly pointed at the apex, which 
is nearly colourless, while the older portions are brownish in colour. Short 
dark-brown rhizoids are present, but these are neither so abundant nor so 
long as Bruchmann figures for B. lunaria. These rhizoids are still less 
conspicuous in older stages, such as that shown in Fig. 2. 
As in the other species of Botrychium the gametophyte is decidedly 
dorsiventral in structure, somewhat flattened, and with a more or less con- 
spicuous median ridge upon which the antheridia are borne. The latter begin 
to develop when the gametophyte is very small, and new ones arise in 
1 Bruchmann: Uber das Prothallium und die Sporenpflanze von Botrychium lunaria, L. Flora, 
1906: Jeffrey, E. C. : The Gametophyte of Botrychium virginianum. Proc. Canad. Inst., 1898, 5 ; 
Campbell : The Eusporangiatae. Carnegie Institution of Washington, Publication No. 140, 1911. 
2 Campbell, Gametophyte and Embryo of Botrychium obliquum , Miihl. Ann. of Bot., xxxv. 
157, 1921. 
mens of Botrychium simplex , Hitch- 
cock, collected by Dr. H. L. Lyon at 
Echo Lake, Minnesota. Natural size. 
C. Leaf segment showing venation, 
x 3. 
