514 Redfern. — On the Course of Absorption and the Position of 
absorbed in appreciable quantity from any concentration. In the cases of 
aniline blue and eosin, equilibrium was attained very rapidly, and no 
curves of absorption could be plotted. 
In Table I the results obtained with neutral red are summarized, and 
the curves of absorption are plotted in Fig. 1. 
In Tables II and III the final external concentrations of all the dyes 
used are given. 
The numbers quoted for the internal concentration are proportional to 
those obtained for the external concentration, and do not represent absolute 
quantities ; they are strictly comparable among themselves, however, as 
approximately the same amount of tissue was used in every case. The 
actual internal concentration is much greater, as the internal volume of 
solution is very small. 
Table I. Absorption of Neutral Red , by Discs of living Carrot Tissue, 
from Solutions of Different Concentrations . 
Duration of 
External concen- 
Relative 
experiment 
tration in 
internal con- 
Weight in grm. 
in hours. 
per cent. 
centration. 
0 
0.1 
0-0 
1.04 
5 
0-08 
0.02 
0.86 
24 
0.06 
0-04 
o-8i 
48 
0-06 
0-04 
0-78 
0 
0.05 
0-0 
0-87 
5 
o-o 35 
0.015 
o -75 
24 
0-03 
0-02 
o-68 
48 
0-03 
0-02 
0.67 
0 
o-or 
0-0 
0-96 
5 
0.007 
0-003 
o -93 
24 
0-004 
0-006 
0.92 
48 
0-002 
0-008 
0-90 
72 
0-0009 
0-0091 
0.90 
96 
0-0007 
0-0093 
0-89 
144 
0-0005 
0.0095 
0-87 
0 
O.OO5 
0-0 
1.06 
5 
0-0025 
C.0025 
1.06 
24 
0-0015 
0.0035 
1*06 
48 
O.OO085 
0.00415 
1.05 
72 
0-0004 
0.0046 
1-06 
96 
0-000225 
0.00475 
1.05 
r 44 
0-000I25 
0.004875 
1.05 
At the end of the experiments in this series a slight yellowish tinge 
was observed ; this showed that the external solution was becoming acid. 
Table II. Absorption of Dye, by living Carrot Tissue, from Solutions 
of Various Concentrations. 
Original Final external coneentration in per cent . — 
external concentration 
Neutral 
Methylene 
Methyl 
Aniline 
Eosin. 
in per cent. 
red. 
blue. 
violet. 
blue. 
0.1 
o-o 6 
0.032 
0.03 
O-I 
O-I 
0.05 
0-03 
0.012 
O-OI 
0.045 
0-045 
O-OI 
0.0005 
0.0008 
0-0018 
c-oo8 
0-0085 
0.005 
0-000125 
0.0003 
O-OOI 
0.003 
0-004 
