Equilibrium in the Intake of Dyes by Discs of Plant Tissue . 5 1 9 
Series 3. Experiments to test the effect of temperature . 
Series of experiments were carried out, in which the bottles containing 
the experimental solutions were kept at 40° C, 20° C., and o° C. The dye 
Fig. 8. Absorption of methylene blue from 0-005 per cent, solution by living 
carrot tissue at different temperatures. 
used was methylene blue, and the experiments were made with living carrot 
tissue. The results are summarized in Table VI and Figs. 7 and 8. 
Similar results were obtained in a series of experiments with methyl violet. 
Table VI. Absorption of Methylene Blue from Solutions of Different' Con- 
centrations by Discs of Carrot Tissue at Different Temperatures. 
Temperature. 
40° C. 
Original external con - External concentration in per cent, after — 
centration in per cent . 
24 hrs. 
48 hrs. 
72 hrs. 
O-I 
0.07 
0.06 
— 
0.05 
0-025 
0-02 
— 
0.0 1 
0.0035 
0-0025 
— 
0.005 
0-002 
0-002 
— 
