282 Brown. — On the Germination and Growth of 
of the carbon dioxide treatment on the germination of fungal spores on 
fruit in store, one would expect a result intermediate between that obtained 
for germination in water and germination in a good nutrient. 
3. The efficiency of the carbon dioxide treatment is enhanced by 
lowering the temperature. Certain cases have been alluded to where 
a moderate concentration (10-20 per cent.) of carbon dioxide at ordinary 
temperature accelerates fungal growth, and though this result was only 
obtained for growth on certain artificial media, it is not at all certain that 
a similar effect might not arise if the storage of certain fruits or vegetables 
were attempted at ordinary temperatures in carbon dioxide. The present 
experiments thus indicate that, as far as concerns fungal attack in the store, 
the gas storage method is to be considered as an aid to the ordinary method 
of cold storage, and as in no way replacing the latter. 
Summary. 
1. Within very wide limits, variation of oxygen pressure has little effect 
on the germination and growth of the ordinary fruit-rot organisms such as 
Botrytis , Fusarium , and Alternaria. 
2. The germination and growth of these organisms is retarded by 
carbon dioxide. The retarding action of carbon dioxide on germination 
and growth is more marked the lower the temperature, the weaker the 
nutrient in which the fungal spores are sown, and, to a less degree, the 
greater the density at which the spores are sown. 
3. The concentration of carbon dioxide which inhibits germination at 
ordinary temperature was determined for the commoner fruit-rot organisms 
both for germination in water and in nutrient. 
4. Graphs and tables are given of the rates of growth of a number 
of fungi at various temperatures and in various concentrations of carbon 
dioxide. 
5. The experimental results indicate that the gas storage method is 
most effectively used in combination with the ordinary cold storage 
method, and that it will give the best results when no attack of the fruit 
has begun previous to storage, and when conditions are such that a minimum 
of nutrient is available to fungal spores on the surface of the fruit. 
Literature cited. 
1. Schneider-Orelli, O. : Versuche liber die Wachstumsbedingungen und Verbreitung der 
Faulnispilze des Lagerobstes. Centr. f. Bakt., 11 xxxii. 161-9, 1912. 
2. Ames, A. : The Temperature Relations of some Fungi causing Storage Rots. Phytopath., v. 1 1- 
19, 1915. 
3. Edgerton, C. W. : Effect of Temperature on Glomerella. Phytopath., v. 247-59, I 9 I 5* 
4. Brooks, C., and Cooley, J. S. : Temperature Relations of Apple-rot Fungi. J. Agr. Res., viii. 
139-64, 1917. 
